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全身麻醉诱导了外围血液中急性无细胞DNA甲基化变化的发生

Wenhua Liang^1,2, Xin Liu³, Zhuxing Chen^1,2

¹Department of Thoracic Surgery and Oncology, The First Affiliated Hospital of Guangzhou Medical University, China National Center for Respiratory Medicine, China State Key Laboratory of Respiratory Disease & National Clinical Research Center for Respiratory Disease, Guangzhou, China.

Journal of thoracic disease

|December 16, 2024

概括

全身麻醉 (GA) 导致细胞自由DNA (cfDNA) 的急性DNA甲基化变化,影响组织修复和预测术后凝血障碍. 这些发现为手术患者提供了潜在的生物标志物.

科学领域:

麻醉学麻醉学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
基因组学就是基因组学.

背景情况:

一般麻醉 (GA) 的不良事件很常见,但潜在的机制仍然不清楚.
研究GA对无细胞DNA (cfDNA) 的DNA甲基化影响对于理解这些影响至关重要.
这项研究的重点是为肺结节进行手术的患者.

研究的目的:

评估GA对cfDNA甲基化概况的总体影响.
为了确定由GA诱导的特定DNA甲基化变化.
探索GA诱导的甲基化变化与术后结果之间的关联.

主要方法:

一项对1006名肺结节患者 (麻醉前后) 的回顾性队列研究.
针对cfDNA的二硫酸盐测序,分析12899个地区和105,844个CpG地点的甲基化概况.
使用随机森林的差异甲基化区域 (DMR) 开发预测模型.

主要成果:

确定了4,562种与发育,细胞粘附,ECM重塑和信号通路 (例如PI3K-AKT,RAS/ERK) 相关的GA诱导的DMR.
使用20个DMR的预测模型准确地预测了手术后凝血指标 (APTT,INR,D-二次体) 和细胞计数 (中性粒细胞,单细胞).
低甲基化cg00232606 (DLX-4基因) 与肺癌患者的存活率较差相关.

结论:

GA诱导了与组织损伤和修复相关的急性DNA甲基化变化.
由GA诱导的甲基化变化与术后凝血功能相关.
这些甲基化变化代表了术后凝血障碍的有希望的预测生物标志物.

关键词:

全身麻醉 (GA) 是一种全身麻醉.没有细胞的DNA (cfDNA)凝血功能障碍凝血功能障碍肺部结节肺部结节甲基化处理的方法

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