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在Lactiplantibacillus plantarum菌株中的CRISPR-Cas系统:使用基因组挖掘方法识别和表征

Mohaddeseh Rostampour¹, Bahman Panahi², Reza Masoumi Jahandizi¹

¹Department of Biology, University of Maragheh, Maragheh, Iran.

Frontiers in microbiology

|December 16, 2024

概括

这项研究分析了675个乳芽植物菌株的CRISPR-Cas系统,发现亚型II-A占主导地位. 这项研究提供了关于CRISPR-Cas进化和菌素向这些细菌的多样性的见解.

科学领域:

微生物学微生物学
遗传学是一个遗传学.
细菌学细菌学是一门学科.

背景情况:

聚集的定期间隔的短平行列重复 (CRISPR) 和与CRISPR相关的 (CAS) 基因形成细菌对细菌菌体的适应性免疫力.
了解工业相关的细菌中的CRISPR-Cas系统,如Lactiplantibacillus plantarum对于控制菌体感染至关重要.

研究的目的:

为了研究CRISPR-Cas系统在675个Lactiplantibacillus plantarum分离物中的多样性,发生和演变.
分析L. plantarum中CRISPR-Cas系统的结构变异,遗传关系和准能力.

主要方法:

来自GenBank. 的675个L. plantarum基因组序列的生物信息分析.
识别和表征CRISPR-Cas系统亚型,重复序列,间隔器和原空间器相邻动机 (PAM).
关于菌体向多样性的CRISPR-CasII-A和IE亚型的比较分析.

主要成果:

在675种L. plantarum菌株中,有143种 (约21.2%) 具有已确认的CRISPR-Cas系统,其中II-A亚型是最常见的.
大约有22%的具有完整CRISPR系统的分离物显示出II-A和IE亚型的同时出现.
与I-E亚型相比,II-A亚型的平均重复长度为36个核酸,并且在向Lactobacillus菌体方面具有更高的多样性.

结论:

这项研究提供了关于L. plantarum的CRISPR-Cas系统流行和演变的全面概述.
这些发现有助于了解菌体耐药性机制,并可以为提高工业发酵中菌体耐药性的策略提供信息.
鉴定到的向多样性为开发基于CRISPR的抗菌药物和推进菌体治疗开辟了道路.

关键词:

这就是CRISPR-Cas.多样性的多样性多样性的多样性进化演化演化演化演化演化演化演化基因组基因组是基因组的组成部分.菌体是一种菌体.

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