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在印度Rewa的牛奶中隔离和鉴定金黄色葡萄球菌 (Staphylococcus aureus)

Shivani Rawat¹, Neeraj Shrivastava¹, Arpita Shrivastav²

¹Department of Veterinary Microbiology, College of Veterinary Science and A.H., Rewa, India.

Indian journal of microbiology

|December 16, 2024

概括

乳制品动物中引起乳腺炎的金黄色葡萄球菌菌株表现出显著的毒性和生物膜形成. 这些乳腺炎病原体的青素和甲素耐药性威胁到治疗疗效和农场经济.

科学领域:

兽医微生物学兽医微生物学
乳制品科学乳制品科学
抗微生物耐药性抗微生物耐药性

背景情况:

乳制品动物的乳腺炎会对牛奶生产,动物福利和农场经济产生负面影响.
黄金葡萄球菌 (Staphylococcus aureus) 是主要的乳腺炎病原体,经常导致难以治疗的感染.

研究的目的:

来自乳制品动物的黄金葡萄球菌菌株的特征.
评估毒性因素,生物膜形成和抗微生物耐药性,包括甲素和青素G耐药性.

主要方法:

100个牛奶样本的细菌培养.
识别和表征金黄色葡萄球菌和耐甲林金黄色葡萄球菌 (MRSA) 隔离物.
评估毒性决定因素,生物膜形成 (Microtiter板法) 和抗菌耐药性 (磁盘扩散试验).

主要成果:

确定了18种金黄色葡萄球菌和6种MRSA分离物.
所有菌株都对毒性决定因素呈阳性;血液溶解不是敏感的毒性指标.
观察到高生物膜形成率 (61.66%S. aureus,83.33%MRSA) 和生物膜基因 (icaA, icaD) 的存在.
超过70%的S. aureus菌株对大多数抗微生物药物敏感,除了青素.
所有MRSA菌株 (100%) 和所有分离物都对青素产生了耐药性.

结论:

抗青素和/或甲素,形成生物膜的金黄色葡萄球菌菌株对乳腺炎治疗构成重大挑战.
这些耐药菌株可能会对治疗结果和奶牛场的经济可行性产生负面影响.
对人口结构和特定抗生素最小抑制度 (MIC) 值进行进一步的研究是有必要的.

关键词:

生物膜是一种生物膜.牛奶就是牛奶,牛奶就是牛奶.乳腺炎是一种乳腺炎.多种药物耐药性概况.黄金葡萄球菌黄金葡萄球菌

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