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在体内双平面3光子显微镜:覆盖老鼠新皮质的深度
Matilda Cloves1, Troy W Margrie1
1The Sainsbury Wellcome Centre for Circuits and Behaviour, University College London, 25 Howland Street, W1T 4JG, London, United Kingdom.
Biomedical optics express
|December 16, 2024
概括
研究人员开发了一种新的成像技术,用于在老鼠大脑中的多个皮质层同时实现单细胞分辨率. 这一突破允许对神经活动和计算进行更深入,更全面的研究.
科学领域:
- 神经科学是一个神经科学.
- 光学成像技术的成像
- 细胞生物学 细胞生物学
背景情况:
- 皮层计算取决于所有层和细胞类型中的神经元活动.
- 三光子显微镜使小鼠的大脑深度成像成为可能,达到所有皮质层.
- 在多个皮质层同时进行成像仍然是一个挑战.
研究的目的:
- 为了在小鼠大脑的多个皮质层中展示同时进行单细胞分辨率成像.
- 为了克服目前用于研究整个皮层柱中的神经活动的成像技术的局限性.
主要方法:
- 组合非单元放大远程聚焦与自适应光学.
- 从两个时间复杂平面实现了单细胞分辨率成像.
- 启用图像平面的距离可以达600微米.
主要成果:
- 从基因定义的细胞类型中同时获得神经元活动的证明.
- 启用在老鼠新皮层柱内的任何一对皮层层的成像.
- 在老鼠大脑深处实现了高分辨率成像.
结论:
- 开发的技术允许在不同类型的细胞和皮质层中同时观察神经元活动,这是前所未有的.
- 这种方法为研究完整大脑中复杂的神经计算提供了一个强大的新工具.
- 光学成像技术的进步对于在系统层面上理解大脑功能至关重要.
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