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结合由 Rhodococcus sp. 进行的定数灭. 在BH4和Acinetobacter sp.中. DKY-1用于控制膜生物反应器中的生物污染

Yanyao Huang¹, Xueman Zheng¹, Yunshi Feng¹

¹Guangdong Provincial Key Laboratory for Plant Epigenetics, Shenzhen Key Laboratory of Marine Bioresources and Ecology, Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China.

Bioresource technology

|December 16, 2024

概括

使用混合细菌珠的新型定数灭 (QQ) 策略有效地打击膜生物反应器 (MBR) 中的生物污染. 这种方法显著延迟了生物污染,减少了微生物生长,为MBR性能提供了有希望的解决方案.

科学领域:

环境科学环境科学
微生物学微生物学
生物技术是生物技术.

背景情况:

生物污染严重损害了膜生物反应器 (MBR) 的效率.
定数灭 (QQ) 是一种破坏微生物通信并防止生物膜形成的策略.
针对物种内部 (N-acyl-homoserine lactones) 和物种间 (autoinducer-2) 信号,提高了QQ的有效性.

研究的目的:

调查一种组合的定数灭 (QQ) 策略,以减轻MBR中的生物污染.
为了比较单种QQ珠子与混合物种QQ珠子的抗生素污染效应.
评估联合QQ策略对生物膜形成和微生物群落结构的影响.

主要方法:

罗多科克菌种 (Rhodococcus sp.) 的固定. BH4 (用于AHL) 和Acinetobacter sp. 这种细菌. DKY-1 (用于AI-2) 在珠子上.
在MBR中应用单种QQ珠和1:1混合QQ珠,以等效的最终QQ剂量.
评估QQ活动,生物膜形成,细胞外聚合物质 (EPS) 和微生物群体组成.

主要成果:

单个和混合的QQ珠都表现出高的定数灭活性.
混合QQ珠显著抑制了微生物生物膜的形成,延迟了生物污染的两倍.
混合QQMBR显示,与单个QQMBR相比,细胞外聚合物质减少,Nitrospira种群减少.

结论:

使用固定细菌的联合QQ策略在缓解MBR生物污染方面非常有效.
破坏AHL和AI-2信号通路提供了优越的抗生素污染性能.
这种方法为提高MBR操作稳定性和效率提供了一个有希望的方法.

关键词:

抗生素污染的产生生物膜是一种生物膜.膜生物反应器是一个膜生物反应器.议员数量的灭.议员数量检测是指对议员数量进行检测.

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