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在试管婴儿患者的阴道与子宫内膜微生物群的不同特征

Alina Polifke¹, Annika von Schwedler^2,3, Rebecca Gulba²

¹dus.ana, Düsseldorf Analytik, Immermannstrasse 65 A, 40210, Düsseldorf, Germany.

Scientific reports

|December 16, 2024

概括

子宫内膜微生物组与IVF患者的阴道微生物组显著不同,不同的细菌组成会影响生殖结果. 子宫内膜微生物组分析可以改善对女性的预后预测,这些妇女经历了植入失败或重复的怀孕损失.

科学领域:

生殖医学生殖医学
微生物组研究微生物组研究
遗传学遗传学是一个

背景情况:

异常的女性生殖道微生物群与妇科疾病有关,并影响生殖结果.
阴道与子宫内膜微生物组的诊断实用性和技术测序变异需要表征.

研究的目的:

在试管婴儿患者的阴道和子宫内膜微生物组进行比较,这些患者有植入失败 (IF) 和/或复发性妊娠丧失 (RPL).
调查微生物组分类方案的重叠情况.
评估16SrRNA测序高变区 (V1-V2与V2-V3) 对生殖器微生物组特征的影响.

主要方法:

追溯性研究比较使用V1-V2 16S rRNA测序的阴道涂抹和子宫内膜活检 (n=71).
分组分析 (n=61) 比较V1-V2和V2-V3序列.
微生物组组成,多样性 (香农) 和分类方案 (社区国家类型 - CST) 的分析.

主要成果:

阴道和子宫内膜微生物组主要由乳酸菌占主导地位,具有共同的物种.
子宫内膜微生物群的多样性高于阴道微生物群 (p=10^-5).
在子宫内膜样本中丰富Corynebacterium,Staphylococcus,Prevotella和Propionibacterium. 在子宫内膜样本中丰富Corynebacterium,Staphylococcus,Prevotella和Propionibacterium.
阴道CST和非乳杆菌主导的子宫内膜微生物组 (9.8%的CST IV与31.0%的NLD) 之间的结果不一致.
V2-V3测序通常与V1-V2一致,在物种检测和CST/NLD率方面存在小差异.

结论:

子宫内膜微生物组与阴道微生物组有很大差异,即使采用了宫横向采样.
鉴定子宫内膜微生物组可以改善在试管婴儿中检测具有不良生殖预后的女性.
16S rRNA测序区域的选择可以影响微生物组的表征.

关键词:

在子宫内膜内,子宫内膜的内膜.生殖器的微生物群.试管婴儿患者试管婴儿患者在RIF中,RIF是RIF.这就是为什么RM是RM.阴道阴道的阴道

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