相关实验视频

Updated: Jun 5, 2025

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

Published on: November 2, 2020

在复杂的体内模型中,CRISPR-StAR能够实现高分辨率的基因查

Esther C H Uijttewaal^1,2, Joonsun Lee^1,2, Annika Charlotte Sell¹

¹Institute of Molecular Biotechnology of the Austrian Academy of Science (IMBA), Dr. Bohr-Gasse 3, Vienna BioCenter (VBC), Vienna, Austria.

Nature biotechnology

|December 16, 2024

概括

通过在细胞后代内部使用内部控制,CRISPR-StAR提高了全基因组遗传查. 这种新的方法提高了数据质量,克服了复杂生物模型的局限性,使基因-表型映射更加稳健.

科学领域:

基因组学就是基因组学.
分子生物学分子生物学
生物技术是生物技术.

背景情况:

聚合的CRISPR-Cas9查使全基因组基因表型映射成为可能.
评估遗传干扰需要大量的细胞来缓解随机漂移.
复杂的模型,如有机体和老鼠异种移植,由于异质性和瓶,存在扩展挑战.

研究的目的:

引入CRISPR-StAR,一种新的查方法,克服了传统的组合基因查的局限性.
为了提高数据质量,生成克隆,单细胞衍生的内在控制.
在复杂的生物系统中确定体内特定的遗传依赖性.

主要方法:

克里斯普尔-StAR激活单导向RNAs (sgRNAs) 在每个细胞后代的一半的后续扩张.
这就产生了克隆细胞群体内的内在控制.
该方法被应用于小鼠黑色素瘤模型的全基因组屏幕.

主要成果:

克里斯普尔-StAR有效地克服了细胞内在和外在的异质性.
该方法减轻了在瓶事件中遇到的遗传漂移.
与传统查方法相比,基准测试表明数据质量优越.

结论:

在复杂和异质模型中,CRISPR-StAR提供了一个强大的解决方案,用于高分辨率的遗传查.
该技术可以更准确地识别基因功能和依赖性.
这一进步有助于研究体内特定的遗传依赖性,特别是在癌症研究中.

更多相关视频

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

Published on: April 8, 2017

相关概念视频

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...