相关实验视频
Updated: Jun 5, 2025

09:33
Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules
Published on: May 12, 2017
14.5K
氧化应激控制了 lncRNA介导的母猪颗粒细胞功能,以 FoxO1 依赖的方式
Wenmin Sheng1, Miaomiao Wang1, Yuqi Li1
1College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
Journal of animal science and biotechnology
|December 16, 2024
概括
氧化应激的影响通过改变花粉状细胞中IncRNA动态,通过FoxO1.1.的介导播种生育能力. 这一途径影响基因表达,影响母猪的生殖健康.
科学领域:
- 生殖生物学 生殖生物学
- 分子内分泌学分子内分泌学
- 基因组学就是基因组学.
背景情况:
- 氧化应激 (OS) 与女性生育能力的降低有关,影响毛囊细胞中的基因表达.
- 目前尚不清楚OS如何影响多组组的确切机制,特别是lncRNAs.
- 这项研究研究了母猪粒状细胞 (sGCs) 中的OS诱导的IncRNome动态和FoxO1.1的作用.
研究的目的:
- 阐明氧化应激在调节母猪颗粒细胞中长非编码RNA (lncRNA) 动态中的作用.
- 为了确定转录因子FoxO1作为这一过程中的关键调解者.
- 了解链接氧化应激,lncRNAs和播种生育的分子途径.
主要方法:
- 生物信息分析用于识别 lncRNA 促进体中的 FoxO 识别元素 (FREs).
- 染色体免疫沉 (ChIP) 和记者测定验证了FoxO1在lncRNA转录中的作用.
- 分析NORSF和CYP19A1基因表达及其与氧化应激和母猪生育能力的关系.
主要成果:
- 氧化应激会诱导sGCs中的IncRNome动态,这在很大程度上取决于FoxO1活动.
- FoxO1直接调节NORSF (参与母猪生育的非编码RNA) 的转录,并抑制CYP19A1,影响雌激素合成.
- 由于促进体变化导致的NORSF转录的失调与母猪生育能力的下降有关.
结论:
- 提出了一种新的信号通路,其中氧化应激通过FoxO1-NORSF轴刺激sGC中的IncRNome动态.
- 这一途径影响sGC功能,并最终影响母猪的生育能力.
- 了解这种机制为改善母猪的生殖结果提供了潜在的目标.
相关概念视频
lncRNA - Long Non-coding RNAs
8.5K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.5K
Inheritance of Chromatin Structures
6.2K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
6.2K
Hormonal Control of the Ovarian Cycle
406
The ovarian cycle is meticulously regulated by the hypothalamic-pituitary-gonadal axis. This cycle orchestrates the release of a mature oocyte, essential for reproduction.
Before puberty, the hypothalamus releases GnRH in a low frequency, low amplitude pulsatile manner. This along with the immature hypothalamic-pituitary-gonadal axis activity, results in low estrogen levels and the absence of a fully functional ovarian cycle. At puberty, GnRH secretion increases in both frequency and...
Before puberty, the hypothalamus releases GnRH in a low frequency, low amplitude pulsatile manner. This along with the immature hypothalamic-pituitary-gonadal axis activity, results in low estrogen levels and the absence of a fully functional ovarian cycle. At puberty, GnRH secretion increases in both frequency and...
406
Regulation of Expression at Multiple Steps
869
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
869
Folliculogenesis
593
Folliculogenesis is the development of ovarian follicles, the specialized structures within the ovarian cortex where oogenesis, or egg development, occurs. This process is essential for female reproductive health and begins during fetal development when primordial follicles are formed. Each primordial follicle comprises a primary oocyte in the center, surrounded by a single layer of squamous pre-granulosa cells. These follicles remain dormant in late prophase I of meiosis until triggered by...
593

