生物发光探针用于检测超氧化物和氧化
Matthew A Larsen1, Mike Valley2, Natasha Karassina2
1Promega Corporation, 277 Granada Drive, San Luis Obispo, California 93401, United States.
ACS chemical biology
|December 17, 2024
概括
新的生物发光探针提供超氧化物 (SO) 和氧化 (NO) 的敏感和特定检测. 这些工具可以直接测量生物样本中的活性氧物种 (ROS),帮助氧化应激研究.
科学领域:
- 生物化学 生物化学
- 细胞生物学 细胞生物学
- 生物技术是生物技术.
背景情况:
- 活性氧物种 (ROS),包括超氧化物 (SO) 和氧化 (NO),对于细胞代谢和信号传递至关重要.
- ROS的失调有助于氧化应激和各种病理.
- 现有的SO和NO检测方法在复杂的生物环境中缺乏灵敏度和特异性.
研究的目的:
- 开发新的生物发光探针,用于直接和选择性定量SO和NO.
- 建立一种敏感和高通量测定方法来测量细胞ROS产量.
主要方法:
- 设计和合成与SO或NO反应的生物发光探针.
- 在ROS检测时,探针会释放d-露西费林类似物.
- 在添加光酶后测量发光,与ROS水平相关.
- 使用无细胞测定和基于巨细胞的细胞研究进行验证.
主要成果:
- 开发的探针显示出对SO和NO的高特异性.
- 在巨细胞中成功检测和量化细胞SO和NO的产生.
- 基于生物发光的测定方法强大,适合用于高通量选.
结论:
- 生物发光探针提供了一种敏感和选择性的方法来测量SO和NO.
- 这些测试有助于直接了解ROS动态和氧化应激.
- 该平台支持ROS相关疾病和生物机制的先进研究.
更多相关视频
08:23Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
Published on: February 16, 2022
3.8K
08:32Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
Published on: March 16, 2017
12.8K
相关概念视频
Labeling DNA Probes
8.1K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.1K
Photoluminescence: Applications
374
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
374
