转录组和蛋白质组分析揭示了JEV感染的PK-15细胞对ferroptosis激活剂和对抗剂的反应中的差异
Xiaolong Zhou1, Yiwei Chen1, Xinyao Kang1
1Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, 666 Wusu Road, Hangzhou 311300, China.
Animals : an open access journal from MDPI
|December 17, 2024
概括
细胞死亡过程 - - 铁亡可以对抗日本脑炎病毒 (JEV) 的扩散. 诱导铁致死有助于控制JEV,为这种动物传播疾病提供了潜在的治疗策略.
科学领域:
- 病毒学 病毒学
- 细胞生物学 细胞生物学
- 免疫学 免疫学 免疫学
背景情况:
- 日本脑炎病毒 (JEV) 引起流行性脑炎B,这是一种影响猪和人类的动物性疾病.
- 细胞防御机制,包括像铁亡这样的受调细胞死亡,对于对抗病毒感染至关重要.
- 在细胞水平上了解宿主-病原体相互作用是开发有效治疗的关键.
研究的目的:
- 调查铁灭菌在日本脑炎病毒 (JEV) 扩散中的作用.
- 为了确定铁灭菌agonists和对抗剂对宿主细胞中JEV复制的作用.
- 为了阐明在JEV感染期间受铁亡影响的分子通路.
主要方法:
- 在实验中,PK15细胞被感染了JEV.
- 细胞被用铁灭酶激动剂 (Erastin) 和抗剂 (SP600125) 治疗.
- 基因表达和蛋白质水平使用转录组和蛋白质组分析进行了分析. 进行了功能丰富分析.
主要成果:
- 铁灭菌激动剂抑制了JEV的扩散,而对抗剂则促进了它的扩散.
- 包括TNF,IL-17,Toll-like受体,PI3K-AKT和化基因通路在内的信号通路受到影响.
- 综合分析确定了31个关键基因,这些基因与JEV感染细胞中的铁亡和炎症有关.
结论:
- 铁死在控制日本脑炎病毒 (JEV) 扩散方面发挥着重要作用.
- 向铁亡途径是对抗JEV的潜在治疗策略.
- 这项研究揭示了铁亡对JEV感染影响的分子机制.
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