谷小麦芽的提取物 限制了由HepG2中过氧化引起的氧化损伤,通过对Redox系统进行上调
Xiaoping Li1, Yuwei Zhang2, Wen Zhao1
1College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710119, China.
Foods (Basel, Switzerland)
|December 17, 2024
概括
麦芽提取物 (TBSE) 通过减少活性氧物种 (ROS) 和增强抗氧化酶来保护肝细胞免受氧化应激. 这种提取物激活Nrf2通路,突出其功能性食品的潜力.
科学领域:
- 生物化学 生物化学
- 细胞生物学 细胞生物学
- 营养科学 营养科学
背景情况:
- 活性氧物种 (ROS) 引起的氧化应激会导致代谢障碍.
- 抗氧化剂可以增强内源抗氧化酶以对抗ROS.
- 地麦芽 (TBS) 富含多,具有潜在的抗氧化特性.
研究的目的:
- 研究麦芽提取物 (TBSE) 对HepG2细胞中过氧化诱导的氧化应激的保护作用和机制.
- 评估TBSE对细胞氧化还原系统和抗氧化酶表达的影响.
主要方法:
- 使用过氧化 (H2O2) 对 HepG2 细胞进行氧化损伤.
- 测量了TBSE对活性氧物种 (ROS) 生产,线粒体膜潜力和细胞抗氧化活性 (CAA) 的影响.
- 分析了抗氧化酶 (SOD,CAT,GST,NQO1,HO-1) 的表达水平和Nrf2的转位.
主要成果:
- 富含六种化合物的TBSE,包括酸和鲁丁,显著抑制了H2O2诱导的ROS过量产生.
- TBSE恢复了线粒体膜潜力,并增加了细胞抗氧化活性 (CAA).
- 通过Nrf2转移到核,TBSE治疗通过Nrf2-Keap1通路的激活提高了抗氧化酶 (SOD,CAT,GST,NQO1,HO-1) 的调节.
结论:
- 麦芽提取物 (TBSE) 有效地保护 HepG2 细胞免受过氧化引起的氧化应激.
- 保护机制涉及Nrf2通路的激活,导致增强的抗氧化剂防御.
- 这些发现支持麦芽对开发功能性食品的潜在健康益处.
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