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在Hydrangea macrophylla中的病毒诱导基因沉默 (VIGS) 和HmF3'5'H的功能分析

Qiyu Yang¹, Youwei Fan¹, Shuwen Luo¹

¹State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.

Plants (Basel, Switzerland)

|December 17, 2024

概括

这项研究建立了一个病毒诱导的基因沉默 (VIGS) 系统在大叶红 (Hydrangea macrophylla) 基因功能研究. 这种新的方法成功地使关键基因沉默,影响了叶子的颜色和花的色素.

科学领域:

植物分子生物学植物分子生物学
园艺科学园艺科学
遗传学遗传学是一个

背景情况:

荷兰花巨型植物对基因功能研究的分子遗传工具有限.
了解基因功能对于改善花颜色等装饰性特征至关重要.

研究的目的:

建立和验证基于烟草虫病毒 (TRV) 的病毒诱导基因沉默 (VIGS) 系统在Hydrangea macrophylla.
在H. macrophylla.中研究植物脱酶 (PDS) 和石墨烯合成酶 (CHS) 基因的功能.
分析F3'5'H基因在氨酸生物合成和花朵颜色决定中的作用.

主要方法:

基于TRV的VIGS系统的开发,用于H. macrophylla的转录后基因沉默.
鉴定和克隆H. macrophylla对PDS,CHS1和F3'5'H基因的正统基因.
TRV构造物的真空透进入H. macrophylla组织培养的幼苗和花朵.
表型分析 (光漂白,色素分析) 和RT-qPCR以确认基因沉默.

主要成果:

TRV-HmPDS静音诱导了叶子中的光漂白,证实了PDS基因的功能.
沉默TRV-HmCHS1降低了反素的产生,并使花朵的分离色变浅.
TRV-HmF3'5'H沉默降低了delphinidin-3-glucoside含量,并将分离体颜色从蓝色转变为粉红色.

结论:

在Hydrangea macrophylla中,TRV-VIGS系统已成功建立并起作用.
这种VIGS系统为H. macrophylla的基因功能分析提供了有效的工具.
这项研究阐明了HmPDS,HmCHS1和HmF3'5'H在红色素和发育中的作用.

关键词:

在HMCHS1中在 HmF3′5′H 的情况下,HmF3′5′H 是在HmPDS中使用.桃花巨型植物 (Hortensia macrophylla) 是一个植物.VIGS VIGS VIGS VIGS VIGS VIGS VIGS VIGS VIGS VIGS VIGS VIGS VIGS VIGS VIGS VIGS VIGS VIGS

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