来自纤维素生物质的纤维素利用的Komagataella phaffii的代谢工程
Jongbeom Park1, Sujeong Park1, Grace Evelina1
1School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea.
Molecules (Basel, Switzerland)
|December 17, 2024
概括
工程酵母 (Komagataella phaffii) 现在可以有效地利用氧化还原酶路径从纤维素生物质中代谢. 这一突破增强了酵母菌的生长和生物质的利用,用于工业应用.
科学领域:
- 生物技术是生物技术.
- 合成生物学 合成生物学
- 微生物工程 微生物工程
背景情况:
- 纤维素生物质水解剂含有葡萄糖和树脂糖,但像Komagataella phaffii这样的酵母与树脂糖利用作斗争.
- 高效的糖代谢对于具有成本效益的生物加工菌素原料至关重要.
研究的目的:
- 通过克西洛斯氧化还原酶途径对Komagataella phaffii进行工程,以增强克西洛斯代谢.
- 优化基因表达以改善生长和生物质转化.
主要方法:
- 通过引入氧化氧化还原酶路径来改造了K. phaffii.
- 利用促进子库对XYL1,XYL2和XYL3基因的表达进行微调.
- 选择了具有强XYL2和较弱XYL1/XYL3促进体表达的菌株.
主要成果:
- 经过工程改造的K. phaffii菌株在kenaf水解剂中实现了显著的生长 (14 g细胞/L).
- 最大生长率达到0.4g细胞/L-h,比原生菌株有17%的改善.
- 证明了成功利用西洛斯,这是生物质价值化的关键步骤.
结论:
- 这项研究是第一个在K. phaffii.中实施氧化氧化还原酶途径的研究.
- 这种工程菌株显示出作为生产酵母蛋白和其他来自纤维素生物质的产品的工业平台的前景.
- 在K. phaffii中优化了糖代谢,为可持续的生物制造开辟了新的途径.
相关概念视频
01:20Fates of Pyruvate
8.3K
Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
8.3K
01:24Other Glycolytic Pathways
The pentose phosphate pathway (PPP) operates in parallel with glycolysis, facilitating the metabolism of both pentoses and glucose. This pathway consists of two distinct phases: the oxidative and non-oxidative phases. While it does not directly generate ATP, the intermediates formed during the process can integrate into glycolysis, contributing to cellular energy metabolism when required.Oxidative Phase: NADPH ProductionThe oxidative phase of the pentose phosphate pathway is primarily...