部分重编程对在培养中经历了复制性衰老的人类介质干细胞产生了再生效应
Julia Ivanova1, Mariia Shorokhova1, Natalia Pugovkina1
1Department of Intracellular Signaling and Transport, Institute of Cytology, Russian Academy of Sciences, Tikhoretskii pr. 4, St. Petersburg 194064, Russia.
International journal of molecular sciences
|December 17, 2024
概括
部分重编程使衰老的人类介质干细胞/干细胞 (MSC) 复原,恢复年轻特征并增强治疗潜力. 然而,无病毒方法对于再生医学的安全应用至关重要.
科学领域:
- 干细胞生物学 干细胞生物学
- 再生医学是一种再生医学.
- 细胞衰老 细胞衰老
背景情况:
- 介质细胞干/干细胞 (MSC) 对于生物医学应用至关重要.
- 长期培养MSC导致复制性衰老,限制其治疗用途.
- 部分重编程为细胞再生提供了一个潜在的解决方案.
研究的目的:
- 调查部分重编程在使衰老的人类子宫内膜MSCs复苏方面的有效性.
- 评估部分重新编程的MSCs的治疗潜力.
- 评估重编程方法的生物安全性.
主要方法:
- 衰老的人类子宫内膜MSCs使用编码Yamanaka因子 (Oct4,Sox2,Klf4,c-Myc) 的仙台病毒载体进行了部分重编程.
- 鉴定包括评估衰老标记 (形态,β-银酸酶,DNA断裂),增殖和DNA损伤反应.
- 使用体外伤口愈合试验评估治疗活性,并测试病毒成分的生物安全性.
主要成果:
- 部分重编程恢复了年轻的形态,减少了衰老标志物,并在MSC中增强了增殖和DNA损伤反应.
- 来自重新编程的MSCs的条件介质与衰老细胞相比,表现出较高的治疗活性.
- 在条件介质中检测到病毒成分,导致红细胞结合,这表明存在生物安全问题.
结论:
- 部分重新编程是一种有希望的策略,用于复苏晚期通道培养的MSCs.
- 使用无病毒协议对于这种复苏技术的安全临床应用至关重要.
相关概念视频
01:29Somatic to iPS Cell Reprogramming
2.2K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.2K
02:15Replicative Cell Senescence
3.6K
Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...
3.6K
01:24Methods of Nuclear Reprogramming
1.8K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
1.8K
01:19Multipotency of Hematopoietic Stem Cells
3.0K
The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
3.0K
01:19Mesenchymal Stem Cells
4.6K
Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
4.6K
01:14Introduction to Nuclear Reprogramming
1.9K
Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
1.9K