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叶绿体ATP合成酶对棉花中反应性氧物种代谢的影响

Li Zhang¹, Panpan Jing¹, Biao Geng¹

¹College of Agriculture, Shanxi Agricultural University, Taigu, Jinzhong 030801, China.

International journal of molecular sciences

|December 17, 2024

概括

反应性氧物种 (ROS) 在棉花中引起男性不育. 这项研究发现,叶绿体基因组中的ATP合成子单元基因 (atpE和atpF) 与ROS代谢有关,为棉花细胞质男性不育提供了洞察力.

科学领域:

植物遗传学植物遗传学
分子生物学分子生物学
农业科学农业科学

背景情况:

棉花中的细胞质男性不育性 (CMS) 与表皮中的异常编程细胞死亡有关,主要由活性氧物种 (ROS) 诱导.
核基因和细胞质基因之间的遗传相互作用是这些异常的基础,强调了解质细胞基因在ROS代谢中的作用的重要性.

研究的目的:

为了研究质细胞基因在棉花细胞质男性不育性 (CMS) 线内的活性氧物种 (ROS) 代谢中的作用,Jin A.
识别与ROS调节相关的叶绿体基因组内的特定基因及其对棉花中男性生育能力的影响.

主要方法:

使用下一代和单分子实时测序来分析棉花CMS系列Jin A (Jin A-CMS) 的叶绿体基因组.
进行了功能性注释和与 *Gossypium hirsutum* хлоропласт基因组进行序列比较.
用基因表达分析 (RT-qPCR) 和基因沉默技术来评估特定基因的功能,包括*atpB*,*atpE*和*atpF*.

主要成果:

锦 A-CMS 叶绿体基因组为 160,042 bp,含有 131 个基因 (112 个功能基因).
与*Gossypium hirsutum*相比,Jin A-CMS中的29个基因显示出单核酸多态,包括ATP合成子单元和光系统复合体.
显著较低的表达 *atpB*, *atpE*, 和 *atpF* 在微胞胎流产期间观察到在金 A-CMS,在F1杂交的表达较高. 抑制*atpE*和*atpF*增加了棉花叶中的ROS水平.

结论:

ATP合成酶子单元基因*atpE*和*atpF*与棉花中的活性氧物种 (ROS) 代谢密切相关.
这些发现为进一步功能分析棉花中的ATP合成酶提供了基础数据,可能为繁殖雄性肥沃或无菌线提供了新的途径.

关键词:

这是ATP合成酶.叶绿体基因组的基因组细胞质男性不孕不育性被编程的细胞死亡.有活性氧物种的反应性氧物种.

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