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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Updated: Jun 4, 2025

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Published on: May 21, 2020

一个改进的RNA提取协议,用于谷全长转录组测序.

Justyna Jazowska¹, Mateusz Przyborowski¹, Marek Wojciechowski¹

¹Plant Breeding and Acclimatization Institute-National Research Institute, Radzików, 05-870 Błonie, Poland.

International journal of molecular sciences

|December 17, 2024

概括

此摘要是机器生成的。

这项研究提出了一种改进的谷物谷物RNA提取方法,克服了污染物带来的挑战. 增强的协议产生了高质量的RNA,适合下游应用,如测序.

关键词:

在IHSP中,它是IHSP.这是一个列表.在 ONTs 上,我们可以看到.在 RIN RIN 中.这是一个RNARNARNARNARNA.这种谷物叫做"secale cereale".谷物谷物谷物谷物麦麦麦麦是一种这是一种粉,粉.

更多相关视频

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis

Published on: January 11, 2017

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

相关实验视频

Last Updated: Jun 4, 2025

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Published on: May 21, 2020

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis

Published on: January 11, 2017

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

科学领域:

植物分子生物学植物分子生物学
生物化学生物化学

背景情况:

从谷物谷物中提取高质量的RNA是很困难的,因为多糖和多.
这些污染物干扰RNA隔离和下游应用.

研究的目的:

开发一种改进的谷物谷物的RNA提取协议.
确保分子研究的高RNA质量和完整性.

主要方法:

一个修改的三反应剂溶液协议与初始净化步骤被开发出来.
修改后的协议与其他五种RNA提取方法进行了比较.
该协议在不同发育阶段的麦谷子上进行了测试.

主要成果:

修改后的协议有效地去除了粉和其他污染物.
实现了高RNA质量 (RIN 9.509.70) 和效率.
提取的RNA适用于mRNA净化和长读转录组测序.

结论:

最初的净化步骤显著改善了从谷物谷物中提取RNA的情况.
这种增强的协议为获得高质量的RNA提供了一种可靠的方法.
该方法适用于麦和潜在的其他谷物种.