在单元分辨率和高分辨率仪器上对总的量化进行敏感LC-MS/MS检测
Jill K Wolken1, Meghan M Peterson1, Wenjing Cao1,2
1University of Wisconsin Hospital and Clinics, Madison, WI 53792, USA.
Journal of clinical medicine
|December 17, 2024
概括
一种新的液体染色学-并联质谱法 (LC-MS/MS) 试验准确地测量了总,提高了临床试验中低度的准确性. 这种方法在多种仪器和样品类型中可靠.
科学领域:
- 临床化学 临床化学
- 分析化学 分析化学
- 质谱测量质量谱测量
背景情况:
- 丸激素是一种重要的雌激素激素,具有广泛的生理作用.
- 现有的免疫测试在低总丸激素度 (<100 ng/dL) 的情况下难以准确,影响女性和儿科的测试.
- 准确测量总丸激素对于各种临床诊断至关重要.
研究的目的:
- 开发和验证一种精确的液体染色学-并联质谱法 (LC-MS/MS) 测定总的方法.
- 为了提高量化低水平的准确性.
- 确保在不同的质谱平台上测试的协调性.
主要方法:
- 血清样本经过稀释和调节.
- 分析物提取使用了支持的液体提取弹.
- 在C18列上进行了染色分离,运行时间为5分钟.
- 根据CLSI指南,对Triple Quad 6500,API 4500和Orbitrap仪器进行了测试验证.
主要成果:
- 实现了0.5 ng/dL的量化极限和2-1200 ng/dL的测量范围.
- 证明了良好的方法验证,当天CV<10%和当天CV<15%.
- 与参考方法相关性很好,在TQ 6500,API 4500和Orbitrap平台上显示一致性.
- 证实了血清分离器和血清激活器管的适用性.
结论:
- 一个强大的和验证的总丸激素LC-MS/MS测定为常规临床使用而建立.
- 该试验提供了准确的量化,特别是在低度下.
- 在三极四极和高分辨率质谱仪之间成功协调,确保了广泛的应用.
相关概念视频
01:33Peptide Identification Using Tandem Mass Spectrometry
6.4K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.4K
01:15High-Resolution Mass Spectrometry (HRMS)
1.2K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
1.2K
01:21Mass Spectrometry: Complex Analysis
714
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
714