通过皮肤输送胰岛素,使用离子液介导的纳米纤维用于糖尿病治疗
Fahmida Habib Nabila1, Rashedul Islam1, Li Yamin1
1Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
ACS biomaterials science & engineering
|December 17, 2024
概括
新的以太和跨以太纳米纤维增强了糖尿病管理的通过皮肤输送胰岛素. 这些生物相容配方显著改善胰岛素透率,并长时间降低血糖水平.
科学领域:
- 纳米技术纳米技术
- 皮肤病学 皮肤病学
- 内分泌学 在内分泌学.
背景情况:
- 通过皮肤输送胰岛素为糖尿病管理提供可控释放,但受到胰岛素大分子量限制,阻碍皮肤透.
- 目前需要高剂量,这影响了商业可行性和患者的坚持.
- 需要新的纳米纤维配方来克服这些障碍.
研究的目的:
- 开发和评估以太体 (ET) 和跨以太体 (TET) 纳米纤维用于增强通过皮肤输送胰岛素.
- 在糖尿病小鼠模型中评估这些配方在改善胰岛素透和控制血糖水平方面的有效性.
主要方法:
- 使用基于脂质的离子液体和各种表面活性剂 (IPM,Tween-80,Span-20) 制备ET和TET纳米纤维.
- 使用动态光散射, ζ-潜在, TEM 和 CLSM 对纳米微粒的表征.
- 在人造人体皮肤和糖尿病小鼠模型上的体外和体内研究,以评估通过皮肤透,生物相容性和血糖降低.
主要成果:
- 稳定的ET和TET纳米微粒具有合适的颗粒大小被成功制成,并保持稳定超过3个月.
- 含异氨基氨酸 (TET1) 的ET和TET配方通过改变角层,显著增强了FITC标记的胰岛素通过小鼠和猪皮肤的透率.
- 纳米微粒证明了生物相容性 (细胞活力>80%) 并显著降低了糖尿病小鼠的血糖水平超过15小时,优于注射.
结论:
- 乙体和跨乙体纳米纤维,特别是TET1,有效地增强了通过皮肤透的胰岛素,并提供持续的血糖控制.
- 这些配方是生物相容和非刺激的,显示商业透皮胰岛素贴片的高潜力.
- 开发的纳米片为糖尿病管理提供了一个有前途,舒适和坚固的替代方案.
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