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在PSAP复合体中的RNPS1控制着细胞循环中的周期性mRNA前剪接

Kazuhiro Fukumura¹, Akio Masuda², Jun-Ichi Takeda²

¹Division of Gene Regulation, Oncology Innovation Center, Fujita Health University, Toyoake, Aichi 470-1192, Japan.

|December 17, 2024

概括

在细胞周期期间,RNPS1蛋白控制周期性RNA剪接. 它在PSAP复合体内发挥作用,调节细胞循环依赖的基因表达,其水平由ubiquitin-proteasome通路控制.

科学领域:

分子生物学分子生物学
细胞生物学细胞生物学
遗传学遗传学是一个

背景情况:

细胞周期进展依赖于受调的基因表达,包括精确的拼接控制.
控制细胞循环依赖拼接的特定拼接因素在很大程度上仍未确定.
极光激酶B (AURKB) 基因表达对细胞分裂至关重要,涉及受调节的剪接.

研究的目的:

为了确定负责AURKB基因细胞周期依赖拼接的拼接因子.
阐明这种拼接因子运作和调节的机制.
确定这种拼接因子在整个细胞周期中调节基因表达中的更广泛作用.

主要方法:

在缺乏RNPS1和PNN的细胞上进行了全转录组测序.
免疫阻塞和蛋白质酶抑制试验被用于评估RNPS1蛋白水平和调节.
分析了包括AURKB内5在内的特定内的拼接模式.

主要成果:

与PNN和SAP18 (PSAP复合体) 复合的RNPS1对于精确拼接特定的内子,特别是AURKB内子5至关重要.
单独或在PSAP复合体中,RNPS1作为内子子集的关键拼接因子.
RNPS1蛋白水平是循环调节的,并通过无素-蛋白酶体通路降低,与周期性拼接事件相关联.

结论:

RNPS1 是一个关键的剪接因子,在细胞周期中调节周期性剪接.
涉及RNPS1的PSAP复合体对于细胞周期依赖的拼接至关重要.
通过ubiquitin-proteasome通路调节RNPS1蛋白质稳定性对于其在循环基因表达中的作用至关重要.

关键词:

细胞生物学细胞生物学细胞生理学细胞生理学分子生物学分子生物学生物分子的特性

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