与人类初级内皮细胞相比,人类iPSC衍生的内皮细胞表现出较低的免疫原性
Haiyan Jia1, Melanie Moore2, Meenu Wadhwa1
1Biotherapeutics and Advanced Therapies, Research and Development, Science and Research Group, Medicines and Healthcare Products Regulatory Agency, Blanche Lane, South Mimms, Potters Bar EN6 3QG, Hertfordshire, UK.
Stem cells international
|December 17, 2024
概括
人类诱导多能干细胞 (iPSC) 衍生的内皮细胞 (ECs) 与原始细胞相比显示出较弱的炎症反应. 这表明iPSC-ECs可能降低了免疫性,提高了它们在血管疾病中用于细胞治疗的安全性.
科学领域:
- 干细胞生物学 干细胞生物学
- 免疫学 免疫学 免疫学
- 血管生物学 血管生物学
- 再生医学是一种再生医学.
背景情况:
- 人类诱导的多能干细胞 (iPSC) 衍生的内皮细胞 (ECs) 具有治疗缺血性血管疾病的潜力.
- 临床应用受到安全问题的阻碍,特别是由于重编程过程导致的异常免疫性.
研究的目的:
- 为了研究iPSC-ECs的免疫表型.
- 在免疫蛋白表达和免疫细胞响应方面,将iPSC-EC与原始人类静脉EC (HUVEC) 进行并排比较.
主要方法:
- 在休息和细胞因子激活的条件下对iPSC-EC和HUVEC的表面蛋白质表达 (MHC I/II类,ICAM-1,E-selectin,VCAM-1) 进行了检查.
- 在5天内监测人类外周血液单核细胞 (PBMCs) 与iPSC-ECs与HUVECs共同培养的增殖.
- 进行混合淋巴细胞反应 (MLR) 来评估T细胞增殖和测量促炎性细胞因子分泌.
主要成果:
- 在iPSC-EC中,MHC I类和ICAM-1表达与HUVEC相似,但TNF-α诱导的E选择蛋白和VCAM-1表达不同.
- 与HUVEC相比,iPSC-ECs在全活性和IFN-γ刺激条件下诱导了PBMC增殖和T细胞激活的减少.
- 与iPSC-EC共培养物分泌的促炎细胞因子水平低于HUVEC共培养物.
结论:
- 与初级EC相比,iPSC-EC通常表现出较弱的炎症免疫反应.
- 这表明潜在的低免疫性,表明在移植环境中免疫排斥的风险降低.
- 这些发现支持iPSC-ECs在血管疾病的细胞疗法中安全有效地使用.
更多相关视频
06:36An In Vitro 3D Model and Computational Pipeline to Quantify the Vasculogenic Potential of iPSC-Derived Endothelial Progenitors
Published on: May 13, 2019
6.0K
09:03Differentiation of Human Induced Pluripotent Stem Cells to Brain Microvascular Endothelial Cell-Like Cells with a Mature Immune Phenotype
Published on: May 19, 2023
2.1K
相关概念视频
01:22iPS Cell Differentiation
2.6K
The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
2.6K
01:06Induced Pluripotent Stem Cells
3.9K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
Somatic...
3.9K