在刺激性肠综合征中,结直肠上皮细胞活性的便生物标志物升高
Per Venge1,2, Valeria Castro Tejera3, Christer Petersson1
1Department of Medical Sciences, Uppsala University and Diagnostics Development, Uppsala, Sweden.
Neurogastroenterology and motility
|December 17, 2024
概括
便生物标志物,如人体脂酶BII前体 (HPLBII-P),人体中性粒细胞卡林 (HNL) 和乙氨基基基衍生神经毒素 (EDN) 在刺激性肠综合征 (IBS) 患者中升高,表明上皮细胞活动超过炎症.
科学领域:
- 胃肠病学 胃肠病学
- 临床生物标志物研究研究
- 功能性胃肠道疾病 功能性胃肠道疾病
背景情况:
- 刺激性肠综合征 (IBS) 是一种普遍存在的功能性胃肠疾病,其病理生理学不清楚.
- 目前的理解缺乏在IBS患者中结肠细胞活动的确切标记.
- 调查便生物标志物提供了一种非侵入性的方法来评估IBS中的结肠细胞功能.
研究的目的:
- 用便生物标志物确定IBS患者的结肠直肠氨基细胞,中性粒细胞和上皮细胞的活动.
- 为了评估这些生物标志物的变化治疗后安慰剂或 mesalazine.
- 为了确定IBS诊断和监测的潜在便标志物.
主要方法:
- 从185名IBS患者和40名健康对照组中收集了便样本.
- 通过ELISA测量了包括calprotectin,eosinophil衍生神经毒素 (EDN),eosinophil阴蛋白 (ECP),人类中性粒细胞脂卡林 (HNL),人类脂酶BII前体 (HPLBII-P) 和髓氧化酶 (MPO) 在内的生物标志物.
- 使用患者日记记录了症状得分.
主要成果:
- 与对照组相比,IBS患者的上皮细胞蛋白HPLBII-P,HNL (pab/765) 和EDN显著增加.
- 中性粒细胞标记物 (calprotectin,MPO,HNL二分体) 和氨基细胞标记物ECP在IBS患者中没有显著增加或减少.
- 梅萨拉治疗导致IBS患者的HNL (pab/765) 和EDN水平增加.
结论:
- 这些发现表明,上皮细胞活动,而不是中性细胞或乙氨基细胞的参与,是IBS的关键因素.
- 在便中增加EDN,HNL (pab/765) 和HPLBII-P可能作为IBS的有价值生物标志物.
- 这些生物标志物可以帮助研究和临床监测刺激性肠综合征.
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