来自在盐度压力下培养的蓝菌株Arthrospira maxima的新型生物活性和功能性外聚糖
Amel Harbaoui1, Nadia Khelifi1,2, Neyssene Aissaoui1
1Department of Chemical and Biological Engineering, Laboratory of Protein Engineering and Bioactive Molecules (LR11ES24), INSAT, University of Carthage, BP 676, 1080, Tunis Cedex, Tunisia.
Bioprocess and biosystems engineering
|December 17, 2024
概括
在盐度压力下,菌外聚糖 (EPS) 的产生增加. 这些多功能生物聚合物表现出用于工业应用的抗菌,抗氧化和乳化特性.
科学领域:
- 微生物学和生物技术:专注于菌和生物聚合物生产.
背景情况:
- 菌外聚糖 (EPS) 对于细胞保护至关重要,并具有独特的特性.
- 最近对EPS的兴趣源于它们在各个领域的多样化应用.
研究的目的:
- 在盐度压力下评估来自Arthrospira maxima的外聚糖 (EPS) 生产.
- 描述产生的EPS的结构和功能特性.
主要方法:
- 在高盐度 (高达40g/L) 下培养Arthrospira maxima,以提高EPS的产量.
- 使用酒精沉,膜透析和冷化来净化EPS.
- 描述涉及大小排除色谱,单糖化物分析和核磁共振 (NMR) 光谱.
主要成果:
- 高盐度显著提高了EPS的产量,产量达到60mg/L.
- 纯化EPS的表面分子量为2.1 × 10^5Da,被确定为异聚合物.
- 核磁共振检测证实了α-anomeric的配置,其中葡萄糖酸,曼诺酸和西洛酸是主要的单糖.
结论:
- 盐度应激是一种有效的方法来促进蓝藻细菌EPS的产生.
- 标志性的EPS表现出对细菌细菌,细菌大麦,大肠杆菌和Klebsiella肺炎的显著抗菌活性.
- 该EPS表现出抗氧化和乳化特性,突出其作为工业用途的多功能生物聚合物的潜力.
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