在Arabidopsis中,DET1通过ubiquitination调节ATAF1抑制的热感应延长
Shuai Yuan1,2, Yan Li1, Yayi Li1
1Solid-State Fermentation Resource Utilization Key Laboratory of Sichuan Province, Department of Agriculture Forestry and Food Engineering, Yibin University, Yibin, China.
Plant cell reports
|December 17, 2024
概括
阿拉比多普西斯转录因子ATAF1通过抑制关键延长基因来负面调节热态生成. 脱乙烯化1 (DET1) 介导的全方位化促进ATAF1降解,微调植物对高温的反应.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 植物开发 植物开发
背景情况:
- 植物表现出热态生成,适应温度升高的适应性反应,以提高适应性.
- 像NAC这样的转录因子 (TF) 调节形态发育和温度应激反应.
- 包括ATAF1/2在内的NACTFs在热态生成中的作用在很大程度上尚未被探索.
研究的目的:
- 为了研究阿拉比多普西斯·塔利亚纳激活因子1 (ATAF1) 在热态生成中的作用.
- 阐明ATAF1调节热敏生长的分子机制.
- 确定参与ATAF1稳定性的翻译后控制的监管机构.
主要方法:
- 在Arabidopsis.中对ATAF1功能进行遗传分析.
- 对热敏基因的基因表达分析.
- 生物化学试验研究ATAF1无处不在和降解.
- 对ATAF1,DET1,PIF4和YUC8.8之间的相互作用进行分析.
主要成果:
- ATAF1作为热态生成的负调节剂,抑制热温度诱导的阴囊延长.
- ATAF1直接抑制关键延长调节器YUCCA 8 (YUC8) 和植物染色相互作用因子4 (PIF4) 的表达.
- 升高的温度促进DET1介导的泛化和ATAF1的降解,减少其抑制作用.
结论:
- 一个DET1-ATAF1-PIF4/YUC8调节模块控制了植物中的热态生成.
- 这个模块微调热敏基因表达,在温暖的条件下可能增加植物适应性.
- ATAF1的稳定性在翻译后的水平上通过在周围环境温度的反应中进行无处不在调节.
更多相关视频
11:33Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer
Published on: October 14, 2022
1.5K
12:36Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo
Published on: January 14, 2016
20.4K
相关概念视频
Transcription
146.6K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
146.6K
TGF - β Signaling Pathway
7.2K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.2K
Tail-anchoring of Proteins in the ER Membrane
3.1K
Tail-anchored, or TA, proteins are estimated to make up to 3-5% of membrane proteins found in the eukaryotic cell. Such proteins have a single transmembrane domain located approximately 30 amino acid residues upstream from the C-terminal end. As a result, the signal recognition particle (SRP) cannot guide a TA protein to the ER membrane for cotranslational insertion. Hence, they are integrated into the ER membrane post-translationally using their C-terminal end as the anchor. TA proteins...
3.1K
Transcription Attenuation in Prokaryotes
15.1K
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
15.1K
