植物对间歇性热应激的反应包括调节mRNA翻译效率的调节
Arnaud Dannfald1,2, Marie-Christine Carpentier1,2, Rémy Merret1,2
1CNRS LGDP-UMR5096, 66860 Perpignan, France.
Plant physiology
|December 17, 2024
概括
植物初始化通过调节翻译来增强热应激的生存能力. 这项研究表明,获得的热耐受性涉及与多体相关的过程,包括共翻译mRNA衰变 (CTRD),这对植物热应激反应至关重要.
科学领域:
- 植物分子生物学 植物分子生物学
- 压力生理学 压力生理学
- 基因调节 基因调节
背景情况:
- 获得的耐热性,或初始化,改善了热应激后的植物生存.
- 之前的研究重点是植物耐热性的转录调节.
- 像翻译和mRNA衰变这样的转录后过程在植物耐热性中的作用仍然不太清楚.
研究的目的:
- 为了调查多体相关过程的参与,特别是翻译和共翻译mRNA衰变 (CTRD),在Arabidopsis热耐受性.
- 为了确定原始化对热应激下一般和特定mRNA翻译效率的影响.
- 阐明CTRD在热应激期间调节基因表达中的作用,特别是与未折叠蛋白反应 (UPR) 相关.
主要方法:
- 阿拉比多普西斯 (Arabidopsis thaliana) 经历了两种热应激模式:仅仅是急性应激,接着是急性应激.
- 在不同的热应激条件下分析mRNA翻译效率和共翻译mRNA衰变 (CTRD).
- 研究未折叠蛋白质响应 (UPR) 路径激活及其与原始化和CTRD.的关系.
主要成果:
- 在急性热应激后,初始化对于恢复植物的一般翻译能力至关重要.
- 热应激降低了非热应激mRNA的翻译效率,同时提高了与热应激相关的mRNA的翻译效率.
- 在急性热应激期间,原始化有利于诱导未折叠蛋白质响应 (UPR) 途径.
- 在缺乏原始化的情况下,ER翻译的mRNA被CTRD所准.
- 在热应激期间,CTRD调节了超过一千个基因,作为一个独立的调节机制.
结论:
- 多种体相关的过程,包括翻译和CTRD,是植物获得热耐性的关键组成部分.
- 开封通过优化转化控制和UPR激活来增强植物对热应激的弹性.
- 在植物热应激反应中,CTRD代表了一个重要的,独立的基因调节机制.
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