鉴定卵细胞细胞质中大型囊泡的组成
Karen L Reader1, Isabella G Pratt1, Georgia L Lawson1
1Department of Pathology, University of Otago, Dunedin, New Zealand.
Reproduction, fertility, and development
|December 17, 2024
概括
卵细胞囊泡储存了对卵细胞质量至关重要的脂质. 它们的尺寸和含量随着成熟而变化,这表明它们对生殖能力的重要性.
科学领域:
- 生殖生物学 生殖生物学
- 细胞生物学 细胞生物学
- 卵细胞的发育和发育.
背景情况:
- 卵细胞囊泡 (真空) 是哺乳动物卵细胞中突出的细胞质成分.
- 它们的体积在成熟过程中减少,在质量较差的卵细胞中较低,这表明它们在卵细胞能力方面发挥了作用.
- 这些细胞器的组成和功能在很大程度上是未知的.
研究的目的:
- 为了确定卵细胞囊泡的脂质含量.
- 调查固定方法对有机细胞大小和保存的影响.
- 为了将囊泡特征与卵细胞质量相关联.
主要方法:
- 羊卵细胞使用离心,活细胞共聚焦显微镜与特定的光染料 (尼罗河红色,LysoTracker,Fluo-4-AM,TMRM) 和传输电子显微镜进行处理.
- 卵细胞被用甲基或甲基固定,用于超结构分析.
- 在实时成像和电子显微镜制剂之间比较了器官分布和大小.
主要成果:
- 尼罗河红色染色显示,卵细胞囊泡含有脂质,与色脂质滴不同.
- 与活细胞成像相比,在电子显微镜制剂中,囊泡和脂质液滴显得明显较小.
- 离心术后的器官分离在成熟 (77%) 与不成熟 (20%) 卵细胞中较高.
结论:
- 卵细胞囊泡是脂质储存器官,对卵细胞的质量和能力至关重要.
- 脂质含量是卵细胞质量的关键因素,需要进一步研究最佳脂肪酸成分以进行体外成熟.
相关概念视频
Overview of Secretory Vesicles
8.4K
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
8.4K
Intralumenal Vesicles and Multivesicular Bodies
3.4K
Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
3.4K
Oogenesis
63.4K
In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...
63.4K


