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类似cGAS的受体驱动了Drosophila中的全身性STING-依赖宿主反应

Xianlong Ai¹, Huimin Deng¹, Xiaoyan Li¹

¹Sino-French Hoffmann Institute, State Key Laboratory of Respiratory Disease, School of Basic Medical Science, Guangzhou Medical University, Guangzhou, China.

|December 17, 2024

概括

类似cGAS受体 (cGLR) -STING通路对于无脊椎动物的抗病毒防御至关重要. 这项研究揭示了它在Drosophila中的系统性作用,影响宿主反应,甚至在病毒感染期间改变睡眠模式.

科学领域:

这是天生的免疫力.
分子生物学分子生物学
病毒学病毒学

背景情况:

干扰素基因 (STING) 的cGAS类受体 (cGLR) -刺激器通路因其在无脊椎动物抗病毒防御中的作用而得到认可.
在无脊椎动物中,其精确的功能和在生物体水平上的系统性影响仍然在很大程度上没有特征.

研究的目的:

在病毒感染期间调查Drosophila中cGAS-STING通路的体内激活和生物的贡献.
阐明这种通路的系统信号机制和生理后果.

主要方法:

使用STING::GFP knockin 记者Drosophila melanogaster可视化通路激活.
给药的循环二核酸3'2'-循环氨酸单酸-氨酸单酸 (cGAMP) 和感染病毒的.
检查了特定组织的记者基因诱导,宫外基因表达和行为变化 (睡眠).

主要成果:

在注射cGAMP时,GFP表达特别在中枢神经系统,中肠,马尔皮吉亚管道和生殖管道中被诱导.
系统性STING信号是由病毒诱导的信号触发的,脂肪体内的异胎cGLR2表达激活了远端组织中的STING.
在交配期间转移了cGLR1/2-依赖的活动,病毒感染以cGLR1/2-和STING-依赖的方式改变了睡眠.

结论:

这种cGAS-STING通路在Drosophila的系统性抗病毒反应中起着重要作用.
这条通路介导感染细胞与其他组织之间的通信,影响宿主生理学.
循环二核酸信号传递是Drosophila天生的免疫和对病毒病原体的反应不可或缺的一部分.

关键词:

3′2′-cGAMPP的 3′2′-cGAMP 的 3′2′-cGAMP 的 3′2′-cGAMP 的 3′2′-cGAMP 的 CP: 免疫学免疫学这种植物是Drosophila.刺痛是一种刺痛.抗病毒免疫力抗病毒免疫力与cGAS类似的受体.睡眠睡眠睡眠睡眠睡眠系统反应是系统的反应.

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