相关实验视频
Updated: Jun 4, 2025

09:05
MicroRNA-based Regulation of Picornavirus Tropism
Published on: February 6, 2017
7.5K
一个以前未知的circRNA通过调节miR-24-3p/KEAP1轴来抑制病毒复制
Haimin Li1, Liuyang Du1, Juan Li1
1MOA Key Laboratory of Animal Virology, Zhejiang University Center for Veterinary Sciences, Hangzhou, China.
PLoS pathogens
|December 17, 2024
概括
伪狂热病毒 (PRV) 感染升级circ29164,一种循环RNA抑制病毒复制. Circ29164与microRNA-24-3p结合,阻止其向KEAP1,从而维持KEAP1水平并限制PRV.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 循环RNAs (circRNAs) 具有多样化的生物学作用,但它们在病毒感染期间的功能在很大程度上仍未被探索.
- 伪狂热病毒 (PRV),一种alphaherpesvirus,作为一个模型来研究宿主circRNA对感染的反应.
研究的目的:
- 识别和描述参与宿主对PRV感染的宿主反应的宿主circRNAs.
- 阐明circ29164影响PRV复制的分子机制.
主要方法:
- 在宿主细胞中的PRV感染模型.
- RNA拉下测试以确定circRNA-miRNA相互作用.
- 对mRNA目标和蛋白相互作用的分析.
- 亡试验 (酶3活性,细胞染色体C释放).
主要成果:
- 病毒感染上调了非编码的circRNAcirc29164.4.
- 发现Circ29164与ssc-miR-24-3p相互作用并具有竞争性结合.
- 这种Ssc-miR-24-3p的向是类ECH相关蛋白1 (KEAP1) 的mRNA.
- 过度表达circ29164或KEAP1诱导了亡并抑制了PRV复制.
结论:
- 发现了一种新型circRNA,circ29164,可以抑制PRV复制.
- Circ29164作为ssc-miR-24-3p的分子海绵,从而稳定KEAP1的表达.
- 这种Keap1的circRNA介导调节会影响PRV感染期间的亡和病毒复制.
相关概念视频
RNA Interference
25.9K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
25.9K
Experimental RNAi
6.0K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.0K
siRNA - Small Interfering RNAs
16.6K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
16.6K
MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
Small interfering RNAs (siRNA)
3.5K
3.5K
piRNA - Piwi-interacting RNAs
6.8K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
6.8K

