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暴露于环境相关度的BP-3在小鼠中通过ER/CCL27/ROS通路诱导了雄性发育生殖毒性

Hong-Mei Li¹, Yan-Rong Gao¹, Qing Chang²

¹The Key Laboratory of Fertility Preservation and Maintenance of the Ministry of Education, Ningxia Medical University, Yinchuan, Ningxia 750004, China; School of Basic Medicine, Ningxia Medical University, Yinchuan, Ningxia 750004, China; School of Public Health, Ningxia Medical University, Yinchuan, Ningxia 750004, China.

Ecotoxicology and environmental safety

|December 17, 2024

概括

低剂量的BP-3暴露会通过破坏精子,丸和丸壁垒来损害男性生殖健康. 这通过雌激素受体CCL27和活性氧物种通路发生.

科学领域:

环境毒理学环境毒理学
生殖毒理学生殖毒理学
内分泌学在内分泌学.

背景情况:

索-3 (BP-3) 是一种常见的紫外线过器,其毒理机制基本上未知.
低剂量BP-3暴露的男性生殖毒性需要进一步阐明.

研究的目的:

在小鼠中研究低剂量BP-3的男性发育生殖毒性.
阐明BP-3诱导的生殖毒性的潜在机制.

主要方法:

小鼠从出生后的第14天或56天开始暴露于BP-3 (2.28和228μg/L).
评估精子参数,水平,血屏障 (BTB) 完整性和氧化应激标志物.
研究了雌激素受体 (ER),化学酶CCL27和活性氧物种 (ROS) 在BP-3毒性的作用.
使用si-CCL27和ROS清理器进行体外TM4细胞检测.

主要成果:

低剂量BP-3显著降低了精子数量,质量和水平.
暴露于BP-3损害了BTB完整性和细胞骨,增加了丸中的氧化应激.
CCL27被BP-3上调,导致氧化应激和毒性.
BP-3与ER直接相互作用,激活ER/CCL27/ROS通路,导致BTB和细胞骨损伤.
ICI 182780 (ER抗剂) 和体外干预对抗了BP-3的影响.

结论:

长时间的低剂量BP-3暴露会导致男性生殖发育毒性.
ER/CCL27/ROS轴是调解BP-3对丸发育的不良影响的关键机制.
这些发现强调了与低剂量BP暴露相关的生殖风险.

关键词:

索芬-3 (BP-3) 是一种血液-丸屏障血液-丸屏障细胞骨架细胞骨架环境相关的度环境相关的度男性生殖毒性有毒性

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