相关实验视频
Updated: May 22, 2026

11:05
Analysis of Oxidative Stress in Zebrafish Embryos
Published on: July 7, 2014
37.1K
6PPD通过触发斑马鱼的氧化应激和铁亡来诱导大脑血管缺陷
Mengzhu Lv1, Xiaoyu Mao2, Zheng Lu1
1Department of Immunology, School of Basic Medicine, Guizhou Medical University, Guiyang 561113, Guizhou, PR China.
The Science of the total environment
|December 17, 2024
概括
通过增加氧化应激,N-(1,3-二甲基) -N'--p-phenylenediamine (6PPD) 破坏斑马鱼的大脑发育和脑血管网络. 用N-乙-L-氨酸 (NAC) 抑制铁亡是一种潜在的治疗策略.
科学领域:
- 发展性毒理学 发展性毒理学
- 神经科学是一个神经科学.
- 环境健康 环境健康
背景情况:
- N-(1,3-dimethylbutyl) -N'-phenyl-p-phenylenediamine (6PPD) 是一种已知对水生有毒性的轮胎抗臭剂.
- 6PPD对脑血管网络发展的影响尚不清楚.
研究的目的:
- 研究6PPD对斑马鱼脑血管发育的影响.
- 阐明6PPD诱导的神经毒性的潜在机制.
主要方法:
- 斑马鱼幼虫暴露于不同度的6PPD.
- 进行了脑特异性转录组分析.
- 评估了反应性氧物种 (ROS) 水平和铁亡标志物.
- 人体血管内皮细胞被用于跨物种验证.
- 进行了铁灭抑制剂N-乙-L-氨酸 (NAC) 的治疗.
主要成果:
- 6PPD暴露不会影响斑马鱼的身体长度或形状,但会导致度依赖的大脑发育缺陷.
- 大脑血管网络被破坏,而干部血管系统没有受到影响.
- 6PPD引发了过度的ROS,并表明大脑中的氧化应激异常.
- 转录组分析揭示了酸代谢的潜在阻塞和铁灭基因的上调调节.
- NAC治疗减轻了氧化损伤,改善了脑血管网络结构.
- 6PPD在人类血管内皮细胞中诱导了类似的氧化应激和缺陷扩张.
结论:
- 6PPD在斑马鱼脑血管网络中引起显著的发育毒性,主要是通过氧化应激和铁亡途径.
- 这些发现突出了物种间保存的毒性机制,表明了对人类血管健康的潜在风险.
- 向铁亡可能为缓解6PPD诱导的血管损伤提供治疗途径.
相关概念视频
Pulmonary Tuberculosis I
Tuberculosis, often called TB, is a contagious illness primarily caused by Mycobacterium tuberculosis. It mainly affects the lung parenchyma but can also impact other body parts.
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...
Pulmonary Tuberculosis II
Tuberculosis, or TB, is a bacterial infectious disease caused by Mycobacterium tuberculosis. While its primary impact is on the lungs, leading to pulmonary tuberculosis, it can also affect various other organs, a condition referred to as extrapulmonary tuberculosis.
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...
Pulmonary Tuberculosis III
Tuberculosis (TB) is a contagious infection primarily affecting the lung parenchyma but which can also affect other body parts. TB can be classified based on disease development, presentation, and the affected anatomical site.
The first classification is based on the development of the disease, and it includes the following categories:
The first classification is based on the development of the disease, and it includes the following categories:
Pulmonary Tuberculosis IV
Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
Pulmonary Tuberculosis V
Medical management of tuberculosis (TB) patients involves a comprehensive approach that includes diagnosis, treatment, and monitoring. The specific strategies can vary depending on the type of tuberculosis (latent or active), the patient's overall health status, and other considerations.
Latent tuberculosis infection occurs when TB bacteria are present in a person's body, but are not causing illness or symptoms. It is not contagious, and preventive treatment is crucial to avoid the progression...
Latent tuberculosis infection occurs when TB bacteria are present in a person's body, but are not causing illness or symptoms. It is not contagious, and preventive treatment is crucial to avoid the progression...

