氨基基基改造通过斑马鱼幼虫的线粒体介导的亡途径加剧了聚乙烯微塑料的发育异常
Jiayi Zhang1, Guocheng Hu2, Hongzhi Guo2
1State Environmental Protection Key Laboratory of Environmental Pollution Health Risk Assessment, South China Institute of Environmental Sciences, Ministry of Ecology and Environment, Guangzhou 510655, China; School of Public Health, China Medical University, Shenyang 110122, China.
The Science of the total environment
|December 17, 2024
概括
氨基基改性微塑料 (PS-NH2) 在斑马鱼中显示出比标准微塑料 (PS-MP) 更大的发育毒性. 这项研究揭示了PS-NH2诱导氧化应激,线粒体功能障碍和亡,影响水生生态系统.
科学领域:
- 环境科学 环境科学
- 毒理学 毒理学 毒理学
- 生态毒理学 生态毒理学
背景情况:
- 微塑料 (MP) 是普遍存在的环境污染物.
- 修改后的MP的毒性机制,特别是它们的表面电荷,尚不清楚.
- 了解MP的毒性对于评估生态风险至关重要.
研究的目的:
- 在斑马鱼中研究氨基基改性聚乙烯微塑料 (PS-NH2) 的发育毒性.
- 阐明毒理学机制,包括氧化应激,线粒体功能障碍和亡.
- 为了比较PS-NH2与传统的聚烯微塑料 (PS-MP) 的毒性.
主要方法:
- 斑马鱼幼虫暴露于环境相关的PS-NH2度 (0.1100μg/L).
- 评估的发展终点:身体长度,心率和自发运动.
- 分析了基因表达 (cat1, sod1, gstr1, nrf2a, nrf2b, HO-1, cox4i1, ndufs1, uqcrc1, caspase-3, bcl-2),氧化应激标志物 (ROS, SOD, CAT, MDA),线粒体功能 (ATP,细胞染色体c, NAD, NADH) 和酶活性.
- 利用分子对接来预测与bcl-2的相互作用.
主要成果:
- 与PS-MPs相比,PS-NH2暴露显著增加了发育毒性.
- 在斑马鱼幼虫中观察到身体长度,心率和自发运动的减少.
- PS-NH2诱导了显著的氧化损伤,线粒体功能障碍和亡.
- 分子对接表明PS-NH2和bcl-2之间的高结合能.
结论:
- 氨基功能化增强了斑马鱼中聚乙烯微塑料的发育毒性.
- 通过氧化应激,线粒体功能障碍和亡,PS-NH2发挥毒性.
- 微塑料的电荷修饰是它们生态毒理影响的关键因素.
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