基于TGase结合代硫酸盐修饰的面团和蛋白质的物理化学特性和结构
Xuyang Ren1, Wanshan Yang1, Huining Zhang1
1College of Food Engineering, Harbin University of Commerce, 150028 Harbin, China.
Food chemistry
|December 17, 2024
概括
使用蛋白质转胺酶 (TGase) 和代硫酸盐 (SMB) 修改,提高了米面的性. 这种处理改善了面团的粘度弹性,使其与小麦面团相比.
科学领域:
- 食品科学 食品科学 食品科学
- 材料科学 是一种材料科学.
背景情况:
- 传统上,米面缺乏小麦面的性,这限制了其在某些食品应用中的使用.
- 研究蛋白质修饰技术,以提高大米蛋白的功能性质.
研究的目的:
- 为了研究蛋白质转胺酶 (TGase) 和硫酸盐 (SMB) 对米面的特性的联合作用.
- 为了分析改性大米蛋白和面团的物理化学和结构变化.
主要方法:
- 使用TGase和SMB对米面的修改.
- 机械分析 (tanδ,纹理分析).
- 物理化学分析 (水结合,粒子大小,二次结构,结合相互作用).
主要成果:
- 修改后的米面表现出类似于小麦面的机械特性 (色,质地).
- 改性大米面团中增加的弱结合水含量.
- 增强了蛋白质结构,增加了α-螺旋和β-转,减少了改性大米蛋白 (MRP) 中的β-片.
- 与未经修改的米蛋白 (URP) 相比,MRP中的,离子和疏水键含量显著更高.
结论:
- TGase和SMB修改有效地改变了大米蛋白的网络结构.
- 组合的修改调节了米面的粘弹性平衡,提高了它的性.
- 这种方法提供了一种可行的方法来增强米面的功能.
更多相关视频
11:25Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins
Published on: October 4, 2017
6.6K
07:43Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice
Published on: October 6, 2020
12.3K
相关概念视频
Plant Breeding and Biotechnology
18.8K
Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
18.8K
Protein and Protein Structure
78.4K
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme...
A protein's shape is critical to its function. For example, an enzyme...
78.4K
Protein Glycosylation
6.8K
Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...
Glycosylation occurs in...
6.8K
