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莱西丁对胡卜素生物可访问性和Caco-2细胞吸收的剂量反应效应
Ziqi Li1, Siqiong Zhong2, Nishita Meshram2
1Department of Food Science and Technology, The Ohio State University, Columbus, OH 43210, United States.
Food chemistry
|December 17, 2024
概括
特定剂量的莱西丁 (1毫克) 显著提高了胡卜素的生物可访问性和细胞吸收. 较高的莱西含量没有改善吸收,并引起负面影响,这表明最佳剂量对生物可用性至关重要.
科学领域:
- 营养科学 营养科学
- 生物化学 生物化学
- 食品科学 食品科学 食品科学
背景情况:
- 胡卜素的生物可用性受到像莱西丁这样的乳化剂的影响,但之前的研究结果不一致.
- 了解莱西的剂量反应关系对于优化胡卜素吸收至关重要.
研究的目的:
- 研究莱西丁对胡卜素生物可访问性和Caco-2细胞吸收的剂量依赖作用.
- 为了确定最佳的莱西丁度,以增强各种类胡卜素的吸收.
主要方法:
- 在体外消化模型中使用β-胡卜素,甘酸盐,黄素,酸盐和酸盐的混合物.
- 使用HPLC-DAD和HPLC-MS/MS. 量化米,米和Caco-2细胞部分中的胡卜素.
- 通过共聚焦显微镜可视化莱西丁-米塞尔相互作用.
主要成果:
- 一毫克的莱西丁剂量大约使胡卜素的生物可访问性翻了一番,并增加了Caco-2细胞对胡卜素 (β-胡卜素,基烯) 的吸收,但并没有增加桑托菲尔 (卢蛋白,泽亚桑丁,阿斯塔桑丁).
- 3毫克或更高的莱西丁剂量没有提高生物可访问性或吸收性,并导致油滴聚合.
- 同焦点显微镜揭示了莱西和菌体之间的特定相互作用.
结论:
- 特定的低剂量莱西丁 (1毫克) 有效地改善了胡卜素的生物可访问性和Caco-2细胞吸收.
- 由于油滴聚合,较高的莱西丁度可能对胡卜素的吸收有害.
- 进一步的人类研究应侧重于有限,优化剂量的莱西丁,以最大限度地提高胡卜素的生物可用性.
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