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低密度脂蛋白受体是flaviviral进入和复制神经元中的重要宿主因素

Meenakshi Bhaskar¹, Anirudh Satheesan¹, Anirban Basu¹

¹National Brain Research Centre, Manesar, Haryana, 122052, India.

Biochemical and biophysical research communications

|December 17, 2024

概括

低密度脂蛋白受体 (LDLR) 是一种新型宿主因子,对于病毒的进入和复制至关重要. 这一发现为开发新的抗病毒疗法来对抗蚊子传播的黄病毒病毒 (如JEV和WNV) 提供了有希望的目标.

科学领域:

病毒学病毒学
分子生物学分子生物学
神经科学是一个神经科学.

背景情况:

弗拉维病毒是蚊子传播的全球重大公共卫生威胁.
控制弗拉维病毒进入和复制的细胞因素尚未完全理解.
低密度脂蛋白受体 (LDLR) 已知用于胆固醇运输,但其在病毒感染中的作用尚未被探索.

研究的目的:

调查LDLR作为宿主因子在日本脑炎病毒 (JEV) 和西尼罗河病毒 (WNV) 复制和进入中的作用.
为了确定LDLR是否作为细胞附着因子来吸收弗拉维病毒.
评估LDLR作为抗病毒研究目标的潜力.

主要方法:

使用了10天大的BALB/c幼和神经元细胞系 (NSC34,HT22).
采用siRNA介导的基因沉默来抑制LDLR表达在体外.
进行了病毒结合,内化和复制试验.
使用抗体中和实验和子宫外LDLR表达.

主要成果:

过渡性LDLR的淘汰显著减少病毒转录和蛋白质.
在LDLR缺乏的细胞中,弗拉维病毒的结合和内化受到了损害.
对LDLR特异性抗体的中和减少了病毒的进入.
宫外的LDLR表达增加了弗拉维病毒的复制.

结论:

LDLR是一种新型宿主因子,对于病毒的进入和复制至关重要.
LDLR作为一种附着因子,促进了JEV和WNV的吸收.
LDLR是开发新抗病毒策略对抗黄病毒病毒的潜在目标.

关键词:

弗拉维病毒 (flaviviruses) 是一种病毒.低密度脂蛋白受体 (LDLR) 是一种低密度脂蛋白受体.运动神经元运动神经元病毒的入口病毒的入口病毒复制是病毒的复制.

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