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EIF4A3-诱导的hsa_circ_0118578表达增强了乳头甲状腺癌的瘤发生

Chan Li¹, Ping Xie¹, Meng Luo¹

¹Department of Tradition Chinese Medicine, Wuhan Third Hospital (Tongren Hospital of Wuhan University), Wuhan, China.

Cancer biotherapy & radiopharmaceuticals

|December 17, 2024

概括

循环RNA的高表达hsa_circ_0118578通过与EIF4A3.3相互作用促进乳头甲状腺癌 (PTC) 恶性病变. 沉默hsa_circ_0118578抑制PTC进展和瘤性,建议它作为一个治疗目标.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

循环RNAs (circRNAs) 与癌症恶性有关.
在乳头甲状腺癌 (PTC) 中hsa_circ_0118578的特定作用仍然在很大程度上未被阐明.
了解新型circRNA对于推进甲状腺癌研究至关重要.

研究的目的:

研究hsa_circ_0118578在PTC细胞恶性瘤中的作用和机制.
为了确定hsa_circ_0118578表达和PTC进展之间的关系.
确定PTC的潜在治疗点.

主要方法:

定量实时聚合酶连锁反应 (qRT-PCR) 用于表达分析.
在体外测试 (Transwell,EDU,伤口愈合) 来评估细胞恶性病变.
在裸体小鼠体内异种移植模型,用于瘤发生性评估.
通过RNA结合蛋白免疫沉来确认分子相互作用.

主要成果:

Hsa_circ_0118578在PTC组织中表达高,与晚期瘤阶段和转移相关.
沉默hsa_circ_0118578显著抑制了PTC细胞的增殖,入侵和迁移.
在体内抑制hsa_circ_0118578减少了瘤生长.
细胞转化启动因子4A3 (EIF4A3) 被确定为一种相互作用蛋白,可以稳定hsa_circ_0118578.

结论:

Upregulated hsa_circ_0118578通过与EIF4A3互动并增强其自身的稳定性来促进PTC的发展.
Hsa_circ_0118578 在PTC中表现出致癌性质.
Hsa_circ_0118578 代表了皮肤状甲状腺癌的潜在诊断和治疗目标.

关键词:

欧洲投资基金4A3A3 hsa_circ_011857878 在线观看在线观看恶性瘤是一种恶性瘤.皮囊性甲状腺癌是什么?

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