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相关概念视频

CRISPR01:59

CRISPR

49.3K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
49.3K

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Fusion of a rice endogenous <i>N</i>-methylpurine DNA glycosylase to a plant adenine base transition editor ABE8e enables A-to-K base editing in rice plants.

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Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
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设计一个强大的Cas12i3变体介导的小麦基因组编辑系统.

Wenxue Wang1,2, Lei Yan1, Jingying Li1,2

  • 1State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences (ICS), Chinese Academy of Agricultural Sciences (CAAS), Beijing, China.

Plant biotechnology journal
|December 18, 2024
PubMed
概括

研究人员为小麦开发了一种新的CRISPR/Cas12i3基因组编辑系统. 这种增强的系统,Opt-T5E-Cas12i3-5M,显著提高了小麦的编辑效率,有助于作物改善.

关键词:
在CRISPR/Cas12i3中使用.在T5外核酶 (T5E) 中.基因组编辑 基因组编辑小麦 (Triticum aestivum L.) 是一种小麦.

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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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科学领域:

  • 植物生物技术 植物生物技术
  • 基因组编辑 基因组编辑
  • 农作物科学 农作物科学

背景情况:

  • 小麦 (Triticum aestivum L.) 是全球重要的粮食作物.
  • 克里斯普尔/Cas12i3提供了诸如较小的蛋白质尺寸和灵活的PAM识别等优势.
  • 之前在小麦中进行Cas12i3中介编辑的尝试受到低效率和作物的六倍形性质的限制.

研究的目的:

  • 为小麦设计一个强大的Cas12i3-5M基因组编辑系统.
  • 通过系统优化,提高六倍体小麦的编辑效率.

主要方法:

  • 在T5外核酶 (T5E) 融合到Cas12i3-5M.
  • 优化crRNA表达策略 (选项).
  • 测试Opt-T5E-Cas12i3-5M系统在三个精英中国小麦品种的四个内源基因上.

主要成果:

  • 在HEK293T细胞中,T5E融合提高了Cas12i3-5M编辑效率,高达3.87倍.
  • 优化的系统 (Opt-T5E-Cas12i3-5M) 在小麦稳定线上实现了1.20至7.95倍的编辑效率,比以前的版本更高.
  • 编辑效率在稳定的小麦系中的目标基因中从60.71%到90.00%不等.

结论:

  • 开发的Opt-T5E-Cas12i3-5M系统是一个强大的小麦基因组编辑工具.
  • 该系统提高了多倍体小麦的编辑效率,克服了以前的局限性.
  • 这项技术有可能加速小麦研究和遗传改进,并可能适用于其他多倍种作物.