相关实验视频

Updated: Jul 7, 2026

Production of E. coli-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope Presentation

Production of E. coli-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope Presentation

Published on: August 21, 2019

采用反向疫苗学方法设计的多表位自我放大mRNASARS-CoV-2疫苗设计

Brigitta Claudia¹, Husna Nugrahapraja^1,2, Ernawati Arifin Giri-Rachman^1,2

¹School of Life Sciences and Technology, Institut Teknologi Bandung, Jalan Ganesa 10, Bandung 40132, Indonesia.

Research in pharmaceutical sciences

|December 18, 2024

概括

这项研究设计了一种针对COVID-19的新型自我放大mRNA (saRNA) 疫苗,通过准SARS-CoV-2尖峰和核囊蛋白. 的疫苗设计显示了有效,低剂量预防COVID-19的潜力.

科学领域:

免疫学免疫学免疫学
疫苗学疫苗学疫苗学
生物信息学是一种生物信息学.

背景情况:

大规模的疫苗分发对于防止SARS-CoV-2传播至关重要.
开发有效的COVID-19疫苗是全球卫生优先事项之一.

研究的目的:

设计一种针对SARS-CoV-2尖峰和核囊蛋白的多表位自我放大mRNA (saRNA) 疫苗.
为印尼人口确定具有高特异性和人口覆盖率的候选疫苗.

主要方法:

从SARS-CoV-2蛋白质中选择的T细胞和B细胞表位基因基于印尼人口的HLA等位基因.
验证了三级结构,并与免疫受体进行了分子对接 (TLR-4,TLR-8,HLA-A*24:02,HLA-DRB1*04:05).
构建了一个编码多表位DNA和VEEV复制机制的重组等离子体,用于saRNA生产.

主要成果:

鉴定了七种B细胞和四种T细胞表位,这些表位是抗原性,保存性,非过敏性,非毒性和水性.
最优的多表位构造 (269 AA) 包括hBD-2辅助剂和PADRE,达到99.26%的人口覆盖率.
分子对接证实了与免疫受体的稳定结合亲和力.

结论:

萨RNA疫苗的in silico设计为预防COVID-19提供了一个有前途的战略.
萨RNA的自我放大性质可以导致减少疫苗剂量要求.
这种多表位saRNA疫苗候选人表明了广泛有效性的潜力.

关键词:

抗原性抗原性的在 COVID-19 疫情中,免疫原性免疫原性免疫原性序列是指一个序列.在 mRNA 疫苗中.

更多相关视频

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain

Published on: July 25, 2022

Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques

Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques

Published on: January 12, 2024

相关概念视频

Cross-reactivity

Cross-reactivity

Cancer Vaccines

Cancer Vaccines

Cancer treatment vaccines are a rapidly evolving field that offers a promising approach to immunotherapy. Unlike traditional vaccines that prevent diseases, cancer treatment vaccines are designed to treat existing cancers by stimulating the immune system to recognize and attack cancer cells.
Cancer vaccines come in two categories: preventive (prophylactic) and treatment (active). Preventive vaccines, such as the Human Papillomavirus (HPV) vaccine, protect against viruses that cause certain...

Vaccine Production

Vaccine Production

Vaccine production involves a sequence of upstream and downstream processes to generate a safe and effective immunological product. It begins with cultivating microorganisms, such as viruses or bacteria, to obtain antigenic material. For viral vaccines, mammalian host cells are grown in bioreactors and subsequently infected with the target virus. The virus replicates within the host cells, which are lysed to release viral particles. This lysate is then clarified through filtration or...

Vaccines

Vaccines

Vaccines are among the most effective tools in preventive medicine, designed to prepare the immune system to recognize and combat infectious agents. By introducing antigens—substances that the immune system identifies as foreign—vaccines stimulate an adaptive immune response that leads to immunological memory. This immunological memory enables the body to mount a faster and more effective response upon future exposures to the actual pathogen.Vaccines can be categorized based on the type of...