在物理实验中对AS2/LOB基因家族的全基因组识别和表达分析
Yuehui Tang1, Xiaohui Wang1, Jiayu Feng1
1College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou, Henan, China.
Frontiers in plant science
|December 18, 2024
概括
物理坚果AS2 / LOB基因对于生长和应激反应至关重要. 这项研究确定了28个JcASL,揭示了它们的多样化表达模式和干旱和盐分耐受性中的作用.
科学领域:
- 植物分子生物学 植物分子生物学
- 基因组学就是基因组学.
- 植物生理学 植物生理学
背景情况:
- AS2/LOB基因是植物中重要的转录因子,调节生长,发育和耐压力.
- 关于天然坚果 (Jatropha curcas) 中的AS2/LOB基因家族的信息有限,尽管它具有经济重要性.
研究的目的:
- 在物理坚果基因组中全面识别和描述AS2/LOB基因家族.
- 在正常和非生物性压力条件下,研究物理学家AS2/LOB基因的表达特征和进化关系.
主要方法:
- 在物理实验中对AS2/LOB基因进行全基因组识别.
- 使用阿拉比多普西斯同类物种的植物遗传学分析.
- 对外子-内子结构,染色体位置,保存域,图案和促进元素的分析.
- 用于表达特征的RNA测序 (RNA-seq) 和定量实时PCR (qRT-PCR).
主要成果:
- 确定了28个AS2/LOB基因 (JcASLs),并分为两组 (I和II).
- 九个染色体的JcASL分布不均,在各种组织 (根,茎,叶,种子) 中观察到差异性表达.
- 两个基因 (JcASL8,JcASL13) 表现出种子特异性表达,16个JcASL对干旱和盐的压力做出了反应.
结论:
- 这项研究提供了对JcASL基因家族的基本理解.
- 这些发现提供了对物理核对非生物压力的反应和其生长调节背后的分子机制的见解.
更多相关视频
11:33Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer
Published on: October 14, 2022
1.5K
10:40Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine
Published on: December 22, 2017
10.4K
相关概念视频
01:33Pleiotropy
39.7K
Pleiotropy is the phenomenon in which a single gene impacts multiple, seemingly unrelated phenotypic traits. For example, defects in the SOX10 gene cause Waardenburg Syndrome Type 4, or WS4, which can cause defects in pigmentation, hearing impairments, and an absence of intestinal contractions necessary for elimination. This diversity of phenotypes results from the expression pattern of SOX10 in early embryonic and fetal development. SOX10 is found in neural crest cells that form melanocytes,...
39.7K
02:24Ribosome Profiling
3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K