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Updated: Jun 4, 2025

Localization of SUMO-modified Proteins Using Fluorescent Sumo-trapping Proteins
06:23

Localization of SUMO-modified Proteins Using Fluorescent Sumo-trapping Proteins

Published on: April 27, 2019

8.4K

设计一个酵母SUMO标签,以消除内部翻译启动

Jamison D Law1,2, Yuan Gao1,3, Vicki H Wysocki1,2,3,4

  • 1Department of Chemistry and Biochemistry, The Ohio State University, Columbus, Ohio, USA.

Protein science : a publication of the Protein Society
|December 18, 2024
PubMed
概括

一种新的SUMO标签变体,SUMONIT,在重组净化过程中防止截断蛋白质的产生. 这种修改通过消除内部翻译启动,确保了全长蛋白质的产量和纯度,特别是在同位体中.

关键词:
这就是所谓的SUMO.闪耀的达尔加诺Ulp1 的使用方法内部翻译的启动原生质谱法本土质谱法.截断的蛋白质是截断的蛋白质.

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