基于EUCAST参考协议的基础上,对14种抗结核药物针对Mycobacterium tuberculosis复合物的常规最低抑制度测定进行微稀释试验
Mikael Mansjö1, Carmen Espinosa-Gongora1,2, Ishak Samanci1
1Public Health Agency of Sweden, Solna, Sweden.
Antimicrobial agents and chemotherapy
|December 18, 2024
概括
这项研究验证了用于结核病药物敏感性测试的汁微稀释试验. 该试验与遗传检测具有很高的一致性,为常规临床使用提供可靠的结果.
科学领域:
- 微生物学 微生物学
- 临床诊断 临床诊断 临床诊断
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 现型药物敏感性测试 (pDST) 对于管理结核病 (TB) 至关重要.
- 针对*Mycobacterium tuberculosis*复合体 (MTBC) 的pDST方法的标准化对于可靠的临床数据至关重要.
- 欧洲抗菌感受性测试委员会 (EUCAST) 提供了针对抗菌感受性测试的参考协议.
研究的目的:
- 为了符合常规MTBC药物敏感性测试 (DST) 的微稀释 (BMD) 试验的资格.
- 评估 BMD 结果与全基因组测序 (WGS) 的基因型 DST (gDST) 结果之间的一致性.
- 为了评估测试对14种抗结核药物的适用性.
主要方法:
- 开发了一种基于EUCAST参考协议的BMD测定.
- 测试了40种MTBC菌株,具有多种药物耐药性.
- 比较BMD衍生的最小抑制度 (MIC) 结果与WGS.的gDST.
主要成果:
- 骨髓质量测试显示,大多数药物与gDST的高度一致 (平均98%).
- 在关键药物中观察到很好的一致性,包括异化,利芬素,贝达奎林和德拉曼尼德.
- 发现了10个差异 (1.8%),主要是接近潜在的临界度.
结论:
- 经过验证的BMD测定为MTBC药物敏感性测试提供了可靠的结果.
- 在常规工作流程中实施这种测试可以提高对MTBC药物耐药性的理解和管理.
- 该测试可适应新的药物建议和当地要求.
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