相关实验视频

Updated: Jun 4, 2025

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Published on: October 6, 2020

在JAZ10中发生的移突变解决了大米的成长与防御困境

Lei-Lei Li¹, Yujie Xiao¹, Baohui Wang²

¹State Key Laboratory of Rice Biology and Breeding, Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China.

Proceedings of the National Academy of Sciences of the United States of America

|December 18, 2024

概括

基因编辑CRISPR创造了一种新的米蛋白,FJ10,可以增强植物生长和害虫抵抗力. 这一发现解开了产量和防御之间的典型权衡,为作物改进提供了新的途径.

科学领域:

植物遗传学植物遗传学
分子生物学分子生物学
农业科学农业科学

背景情况:

通过CRISPR-Cas9基因组编辑,可以在植物中进行有针对性的基因修改.
斯酸盐 (JA) 信号通路调节植物生长和防御反应.
非同源端结合 (NHEJ) 途径是CRISPR诱导突变的常见机制.

研究的目的:

为了研究CRISPR-Cas9编辑对莉花信号基因OsJAZ10.Z的影响.
通过OsJAZ10编辑生成的新型移蛋白 (FJ10) 的特征.
探索FJ10在改善水生长和防御方面的潜力.

主要方法:

使用CRISPR-Cas9基因编辑,在大米中的OsJAZ10基因中引入INDEL.
确定了框架转移突变,导致了新的FJ10蛋白.
进行过度表达研究和蛋白质相互作用分析 (使用OsSLR1和OsFBK16).
与表达FJ10的植物进行了实地实验.

主要成果:

对OsJAZ10的CRISPR-Cas9编辑没有影响正规JA信号.
一个框架转移突变产生了FJ10蛋白,增强了大米生长和棕色植物的抵抗力.
FJ10与OsSLR1和OsFBK16相互作用,破坏它们在生长和防御调节中的功能.
在实地试验中,表达FJ10的植物在没有损害防御的情况下表现出更好的产量.

结论:

新型的FJ10蛋白,来自CRISPR编辑的OsJAZ10,提供了一种提高大米产量和害虫抗性的策略.
通过调节吉伯雷林和红素通路,FJ10解开了生长防御权衡.
这项研究为开发具有增强农业特征的改良作物品种开辟了可能性.

关键词:

棕色的植物跳框架移位突变突变是什么?增长与国防之间的权衡.雅斯蒙酸是一种酸.米米饭米饭米饭米饭.

更多相关视频

Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer

Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer

Published on: October 14, 2022

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Published on: September 2, 2019

相关概念视频

Transgenic Plants

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

Cell Signaling in Plants

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...

Meristems and Plant Growth

Meristems and Plant Growth

Plants grow throughout their lives; this is called indeterminate growth, and it distinguishes plants from most animals. Although certain parts of plants stop growing (e.g., leaves and flowers), others grow continuously—like roots and stems.

Cells Coordinate Growth and Proliferation

Cells Coordinate Growth and Proliferation

Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...

The Ras Gene

The Ras Gene

The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...

Mismatch Repair

Mismatch Repair

Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...