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复杂的生物样本中含有的纳米粒子的高效提取和分析方法,通过使用SP-ICP-MS消除"虚假"干扰

Qinfei Zhou¹, Lihong Liu², Junhui Zhang¹

¹State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China; College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, 100049, China.

|December 18, 2024

概括

一种新方法有效地提取和分析生物样本中的含金属纳米粒子 (MNP). 这种技术克服了干扰,使组织中准确检测到纳米粒子 (PbNP),这对于环境和毒理学研究至关重要.

科学领域:

环境科学环境科学
分析化学分析化学
毒理学毒理学毒理学

背景情况:

含有金属的纳米粒子 (MNP) 在环境和生物中广泛存在.
它们在生物样本中的提取和分析由于其他金属物种和复杂矩阵的干扰而存在重大挑战.
了解MNP的命运和毒性需要准确的分析方法.

研究的目的:

建立一个有效和准确的方法来提取和分析生物样本中的MNP.
在MNP分析过程中消除金属离子和生物矩阵的干扰.
调查纳米颗粒 (PbNPs) 在暴露于环境来源的生物体中的发生和分布.

主要方法:

开发了一种提取方法,与单颗粒感应合等离子体质谱学 (SP-ICP-MS) 相结合.
在四甲基氧化物 (TMAH) 提取过程中使用乙烯基二胺酸 (EDTA) 和超声波处理,以减轻干扰.
应用该方法来分析在发电厂附近收集的生物组织.

主要成果:

已确定的方法有效地消除了离子的干扰信号,防止了假阳性和过高估计纳米粒子 (PbNP).
在各种生物基质中观察到PbNP的干扰信号,包括肝脏,大脑,胆汁,肠道和胃.
在胃,肠道和肝脏组织中成功提取,表征和定量PbNP,这表明口服暴露和潜在的转移.

结论:

开发的方法为有效提取和精确分析生物样本中MNP提供了可靠和强大的工具.
这种技术对于调查MNP在环境和生物系统中的发生,命运和毒性至关重要.
这些发现突显了暴露生物中PbNP的存在,强调了进一步进行毒理学评估的必要性.

关键词:

生物矩阵是生物矩阵.提取提取提取干扰干扰干扰干扰含有的纳米粒子.在SP-ICP-MS中使用SP-ICP-MS.

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