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辐射诱导的旁观者效应在海星 (Patiria pectinifera) 卵细胞中

Haruki Chiba¹, Hinata Horikawa¹, Valerie Swee Ting Goh²

¹Department of Clinical Laboratory Sciences, School of Health Sciences, Fukushima Medical University, Fukushima, 960-8516, Japan.

Radiation research

|December 18, 2024

概括

辐射诱导的旁观者效应 (RIBE) 在海星中得到保留. 未被辐射的海星卵细胞在与被辐射的卵细胞共同培养时显示出细胞死亡的增加,这表明辐射反应的进化途径得到保护.

科学领域:

放射生物学的放射生物学
发展生物学发展生物学
海洋生物学海洋生物学

背景情况:

电离辐射可以在培养的哺乳动物细胞中诱导旁观者效应 (RIBE).
在无脊椎动物中,RIBE的进化保护在很大程度上仍未被探索.

研究的目的:

研究无脊椎动物中辐射诱导的旁观者效应 (RIBE) 的存在和进化保存.
为了确定海星卵细胞的X射线辐射是否会在共同培养的未被辐射的卵细胞中诱导旁观者效应.

主要方法:

海星 (Patiria pectinifera) 卵子与X射线照射的卵子 (0,2和4 Gy) 共同培养.
使用形态异常评分和TUNEL测定来评估未经辐射的卵细胞中的细胞死亡.
还评估了受辐射卵细胞的条件介质对未受辐射卵细胞的影响.

主要成果:

与2Gy或4Gy辐射的卵细胞共培养的未经辐射的卵细胞显示细胞死亡频率显著增加.
在暴露于辐射诱导的旁观者信号的未被辐射的卵细胞中,形态异常和TUNEL阳性细胞升高.
受到4Gy辐射的卵细胞的海水条件诱导了细胞死亡和未被辐射的卵细胞中的TUNEL阳性细胞.

结论:

辐射诱导的旁观者效应 (RIBE) 存在于海星 (Patiria pectinifera) 卵细胞中.
这些发现表明,在无脊椎动物中,RIBE在进化过程中得到了保存,并超越了哺乳动物细胞系.
这项研究在海洋无脊椎动物模型中提供了辐射诱导损伤细胞间通信的证据.

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