在使用葡萄糖作为唯一的碳源的De novo2'-fucosyllactose生物合成中,使用多种工程 Bacillus subtilis 生物合成
Quanwei Zhang1, Xianhao Xu2, Wei Zhang2
1Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China; Science Center for Future Foods, Jiangnan University, Wuxi, 214122, China; Institute of Future Food Technology, JITRI, Yixing, 214200, China.
Metabolic engineering
|December 18, 2024
概括
工程 Bacillus subtilis 有效地生产2'-Fucosyllactose (2'-FL) 仅使用葡萄糖. 这种新的方法大大降低了这种重要的母乳寡糖的生产成本.
科学领域:
- 生物技术是生物技术.
- 合成生物学 合成生物学
- 微生物工程 微生物工程
背景情况:
- 2 -糖乳糖 (2 -FL) 是一种关键的母乳寡糖,对婴儿健康至关重要.
- 由于依赖多个碳来源,目前的生产方法是昂贵的.
- 开发具有成本效益的2FL生产对于工业应用至关重要.
研究的目的:
- 为了设计一种Bacillus subtilis菌株,以使用葡萄糖作为唯一的碳来源,高效地生产2 - FL.
- 通过代谢工程和遗传电路构造来增强2-FL标位.
- 建立一个低成本的工业2FL制造业的基础.
主要方法:
- 使用Neisseria meningitidis基因构建了一个乳糖生物合成模块.
- 综合乳糖和GDP-L-糖生物合成途径.
- 引入了一个外源的非酸化运输系统.
- 开发了一种对乳糖敏感的基因电路,用于动态代谢调节.
主要成果:
- 通过使用葡萄糖,达到2.53 g/L的初始2 -FL标位.
- 通过增强细胞内葡萄糖供应,提升标位至4.94g/L.
- 动态调节的菌株在摇瓶中产生了9.67g/L (107%的改善).
- 在3L生物反应器中达到30.1g/L标位和0.15g/g收益率.
结论:
- 成功设计了B. subtilis用于从葡萄糖中产生高产量的2-FL.
- 证明了多维工程策略的有效性.
- 铺平了2 -FL的经济高效的工业生产的道路.
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