探索解酵母的多样性:在葡萄酒制造中的应用中,向有效的多聚氨酸酶生产者发展
Mehmet Gazaloğlu1,2, Carole Camarasa2, Elke Nevoigt1
1School of Science, Constructor University, Campus Ring 1, 28759 Bremen, Germany.
FEMS yeast research
|December 18, 2024
概括
这项研究引入了一种新方法,以寻找有效生产聚氨酸酶 (PGase) 的酵母菌株,该酶对于葡萄酒制造等工业应用中的pectin分解至关重要. 改进的查成功地确定了表现最佳的酵母菌株的乳酸分解活性.
科学领域:
- 昆虫学 昆虫学是一门关于昆虫的学科.
- 工业微生物学 工业微生物学
- 食品科学 食品科学 食品科学
背景情况:
- 来自酵母的解酶提供了重要的工业潜力,特别是在酒方面.
- 目前用于选乳酸酵母菌株的选方法,特别是对聚氨酸酶 (PGase) 分泌的选方法,在预测工业性能方面是有限的.
- 可靠地识别高性能酵母菌株对于优化富含点氨酸的工业流程至关重要.
研究的目的:
- 开发和验证一种新的选方法,用于在工业条件下识别具有高聚甲氨酶 (PGase) 分泌能力的酵母菌株.
- 解决传统选方法在预测工业酶生产方面的局限性.
- 发现和描述强效的解酵母菌株,用于葡萄酒制造和其他加工行业的应用.
主要方法:
- 分析了来自17个物种的512个酵母菌株的PGase分泌,使用酒作为模型系统.
- 采用传统的光测试在酵母--乳糖 (YPD) 中介,然后在20°C的合成葡萄汁中介与聚氨酸 (PG) 进行化.
- 利用一种新开发的点-测定方法,精确量化液体培养物中PGase活性,用于高性能菌株.
主要成果:
- 最初的光环测定确定了118个PGase阳性菌株,分布在9个属.
- 在合成葡萄汁介质上进行的查将有前途的菌株数量降至35种,属于五个属 (Kluyveromyces,Cryptococcus,Pichia,Torulaspora,Rhodotorula).
- 酸测定突出了来自Kluyveromyces和Cryptococcus物种的菌株,因为它们具有卓越的解活性.
结论:
- 开发的选方法有效地识别出用于工业应用的非常有前途的乳清酵母菌株.
- 方法上的进步使得在与工业过程相关的条件下能够更准确地预测PGase分泌.
- 这种方法为在葡萄酒制造和其他加工富含点氨酸材料的工业中利用点氨酸酵母开辟了新的可能性.
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