相关实验视频

Updated: Jun 4, 2025

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Published on: May 6, 2019

在急性髓性白血病中增加IDO表达和调节性T细胞:对免疫逃脱和治疗向的影响

Raziyeh Hakak¹, Behzad Poopak^2,3, Ahmad Majd¹

¹Department of Cellular and Molecular Biology, Faculty of Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran.

|December 18, 2024

概括

调节性T细胞 (Tregs) 和胺二氧化酶 (IDO) 在急性髓性白血病 (AML) 中升高. 与化疗相结合的IDO抑制可以克服AML患者的免疫逃逸.

科学领域:

免疫学免疫学免疫学
在瘤学瘤学.
生物化学生化学

背景情况:

急性髓性白血病 (AML) 是一种异质的血液性恶性瘤.
免疫失调在AML的发病和进展中起着至关重要的作用.
调节性T细胞 (Tregs) 和氨酸二氧化酶 (IDO) 是免疫抑制的关键参与者.

研究的目的:

为了确定调节性T细胞 (Tregs) (CD4+/FOXP3+) 和氨酸二氧化酶 (IDO) 在AML患者表达的频率.
调查Treg频率,IDO表达和AML之间的关系.
评估潜在的治疗策略,针对在AML中的IDO.

主要方法:

横截面病例控制研究,涉及15名AML患者和20名健康对照.
骨髓样本使用流动细胞计,实时PCR和西部涂抹分析.
对Treg细胞,IDO表达和TGF-β水平的量化.

主要成果:

与对照组相比,AML患者的T-辅助 (Th) 淋巴细胞中的Treg百分比显著高于AML患者 (p<0.05).
在AML患者中,IDO表达和蛋白质水平显著升高 (分别为p=0.004和p<0.001).
在AML患者的Th淋巴细胞中,IDO表达和Treg百分比之间发现了正相关性 (r=0.637,p=0.003).
在AML患者中观察到TGF-β水平升高 (p<0.01).

结论:

患有AML的患者表现出IDO表达和Tregs的水平增加.
IDO抑制,结合化疗,是克服AML免疫逃生机制的有希望的策略.
准IDO可能会增强抗白血病免疫反应.

关键词:

急性髓性白血病是什么?急性髓性白血病是什么?流动细胞计量流动细胞计量印度勒胺2,3-二氧化基酶.实时的聚合酶连锁反应.监管T细胞是T调控细胞.

更多相关视频

Functional Characterization of Regulatory Macrophages That Inhibit Graft-reactive Immunity

Functional Characterization of Regulatory Macrophages That Inhibit Graft-reactive Immunity

Published on: June 7, 2017

Pooled shRNA Library Screening to Identify Factors that Modulate a Drug Resistance Phenotype

Pooled shRNA Library Screening to Identify Factors that Modulate a Drug Resistance Phenotype

Published on: June 17, 2022

相关概念视频

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...

Tumor Immunotherapy

Tumor Immunotherapy

Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.