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一个染色体尺度参考基因组的Banna微型杂交猪

Hong-Man Chen^1,2,3, Kai-Xiang Xu^1,3, Chen Yan⁴

¹Yunnan Province Key Laboratory for Porcine Gene Editing and Xenotransplantation, Yunnan Agricultural University, Kunming, 650201, China.

Scientific data

|December 18, 2024

概括

对Banna小型猪 (BN) 进行了新的参考基因组测序,改善了其在生物医学研究和异种移植中的使用. 这种高质量的基因组提供了关于近亲繁殖的见解,并增强了BN猪模型.

科学领域:

基因组学就是基因组学.
动物模型动物模型
生物医学研究生物医学研究

背景情况:

班纳微型猪 (BN) 由于其遗传稳定性,是研究的有价值的杂交品种.
在BN和其Diannan微型猪 (DN) 的起源之间存在显著的遗传差异.
缺乏BN参考基因组阻碍了全面的基因组研究.

研究的目的:

为Banna小型猪 (BN) 产生高质量的染色体尺度参考基因组.
提供基因组资源,以了解近亲繁殖,并推进在生物医学研究和异种移植中利用BN猪.

主要方法:

使用了PacBio的HiFi和Hi-C测序技术.
进行了基因组组装和注释.
质量评估包括BUSCO和OMArk分数.

主要成果:

一个2.66 Gb的染色体规模的BN基因组被装配到一个143.60 Mb的N50支架上.
97.59%的序列固定在染色体上,具有高的BUSCO (96.30%) 和OMArk (99.58%的蛋白质完整性) 评分.
基因组注释确定了19756个编码蛋白质的基因和7207个非编码RNA,其中48.49%的重复内容.

结论:

染色体尺度BN基因组代表了与现有的猪基因基因相比的重大进步.
这种基因组资源有助于对近亲繁殖的遗传基础进行更深入的研究.
改进的基因组数据将加强BN猪在生物医学研究和异种移植中的应用.

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