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在Litopenaeus vannamei的Ecytonucleospora hepatopenaei (EHP) 疾病患病率和死亡率:印度东部养殖场的一项比较研究

Vikash Kumar¹, Basanta Kumar Das², Souvik Dhar¹

¹Aquatic Environmental Biotechnology (AEB) Division, ICAR-Central Inland Fisheries Research Institute (CIFRI), Barrackpore, 700120, India.

BMC microbiology

|December 18, 2024

概括

甲状腺核 (Ecytonucleospora hepatopenaei,简称EHP) 导致了养殖的重大损失. 这项研究证实了印度养殖场的EHP,揭示了分子变化和与Vibrio物种的共感染,进步了对这种寄生虫的理解.

科学领域:

水产养殖是水产养殖的一种方式.
鱼类病理学鱼类病理学
微生物学微生物学

背景情况:

甲状腺核 (Ecytonucleospora hepatopenaei,简称EHP) 是一种微型虫寄生虫,影响养殖.
在水产养殖行业中,EHP会导致生长减缓和重大经济损失.

研究的目的:

为了在印度西孟加拉邦的养场中分子确认EHP的存在.
研究EHP感染对的分子和生态影响.

主要方法:

嵌套PCR用于在组织,便,料和环境样本中检测EHP.
进行了组织病理学,基因转录分析 (ProPO,Hsp70,α2-宏球蛋白,LGBP,PXN,Integrin ß) 和肠道微生物群分析.
使用Artemia franciscana进行了体内生存测试,以确定共致病原体.

主要成果:

在西孟加拉邦农场的有症状的中证实了EHP.
组织病理学显示,受感染的的成熟子和死体管道.
基因表达分析显示,PROPO,Hsp70和α2-宏球蛋白的显著上调,以及LGBP,PXN和Integrinβ的下调.
乙肝病毒感染改变了肠道细菌群落,并与致病性Vibrio物种 (V. parahaemolyticus,V. harveyi,V. campbellii) 有关.

结论:

这项研究证实了印度的EHP,详细说明了其病理和分子效应.
在有症状的中与Vibrio物种的同时感染表明了复杂的疾病病因.
这些发现提高了对EHP病理生物学的理解,这对健康管理至关重要.

关键词:

利托佩内亚斯 (Litopenaeus vannamei) 是一个水族群.在EHP中使用EHP.基因表达基因表达基因表达肠道细菌是什么肠道细菌是什么在道德上,道德是道德.

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