由于不平衡的营养和FSH诱导的卵巢过度刺激,在绵羊的状子宫内膜中发生的转录基因变化
Özlem Bedir1,2, Miguel Tavares Pereira1, Hubert Rehrauer3
1Institute of Veterinary Anatomy, Vetsuisse Faculty, University of Zurich (UZH), Winterthurerstrasse 260, Zürich, CH-8057, Switzerland.
BMC genomics
|December 18, 2024
概括
营养状况和超排卵 (SOV) 影响羊子宫基因表达. SOV对子宫内膜的作用取决于饮食,影响子宫的受容性.
科学领域:
- 生殖生物学 生殖生物学
- 基因组学就是基因组学.
- 动物科学动物科学
背景情况:
- 外源性淋巴激素和不均衡的饮食会影响子宫功能.
- 在羊群中,卵泡刺激激素 (FSH) 诱导的超排卵 (SOV) 改变了植入基因表达.
- 深度RNA测序 (RNA-Seq) 用于研究这些对子宫内膜的影响.
研究的目的:
- 阐明营养状况和超排卵 (SOV) 对绵羊状子宫内膜转录组的影响.
- 为了确定与饮食和FSH治疗相关的差异表达基因 (DEGs).
- 了解身体状况如何影响SOV对子宫基因表达的影响.
主要方法:
- 雌羊被分配到控制养 (CF),过量养 (OF) 或不足养 (UF) 组.
- 每个营养组分为超排卵 (SOV) 或盐水控制 (CONT) 治疗.
- 乳腺内膜样本是在期的第10天 (CF_CONT的第5天) 采集的,用于RNA-Seq分析.
主要成果:
- 在第5天和第10天,CF_CONT组之间确定了1484个DEG,与免疫活性,新陈代谢和增殖有关.
- 在CONT动物中不平衡的营养 (OF,UF) 影响了细胞粘附,分化,免疫反应和血管生成.
- 在CF (1374) 中,FSH的转移效应产生了更多的DEG,而不是OF (168) 或UF (18) 动物,主要影响细胞周期和荷尔蒙敏感性.
结论:
- 超排卵 (SOV) 以身体状况依赖的方式影响 caruncular 转录组.
- FSH对基因表达的影响是多向的,并且根据营养状况而异.
- Ovine caruncular homeostasis 和子宫受体性都受到身体状况和SOV的影响.
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