该AxBioTick研究 - 免疫基因表达签名在被Borrelia感染的人类皮肤被咬伤的感染者与非感染的
Nellie Carlströmer Berthén1, Samuel Cronhjort2, Marika Nordberg3,4
1The Borrelia Research Group of the Åland Islands, the Åland Islands, Mariehamn, Finland. nellie@bimelix.ax.
BMC infectious diseases
|December 18, 2024
概括
皮肤暴露在Borrelia感染的身上会触发免疫基因表达的增加,即使没有可检测的BorreliaDNA. 这表明子和螺旋虫对免疫系统的影响,有助于未来诊断菌感染.
科学领域:
- 免疫学 免疫学 免疫学
- 基因组学就是基因组学.
- 传播的疾病 传播的疾病
背景情况:
- 由Borrelia burgdorferi sensu lato引起的Borrelia感染是由北欧普遍存在的Ixodes ricinus传播的.
- 确立Borrelia感染涉及螺旋体传播和宿主的皮肤免疫反应后咬.
研究的目的:
- 调查咬后皮肤中的局部免疫反应.
- 评估Borrelia对宿主免疫系统的潜在直接影响.
- 分析暴露在受感染的皮肤中的基因表达与未受感染的皮肤.
主要方法:
- 咬后7-10天收集的皮肤活检.
- 使用实时PCR检测Borrelia spp. 和其他传播的病原体在和活检中.
- 进行了用于皮肤转录基因组分析的RNA测序,重点是免疫系统调节.
- 对转录组数据进行了丰富分析.
主要成果:
- 暴露在Borrelia阳性的皮肤活检显示,免疫相关基因的表达显著更高.
- 上调的基因包括调节T细胞和巨细胞激活的细胞因子,促炎因素和用于病原体识别的Toll类受体 (TLR2,3,7).
- 在分析的皮肤活检中没有检测到Borrelia DNA.
结论:
- 暴露于Borrelia的皮肤中的高调基因表达表明和螺旋虫的免疫影响.
- 鉴定皮肤中波雷利亚相关的基因表达特征可以促进诊断.
- 这项研究提高了对Borrelia感染发展及其表现的理解.
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