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老年大脑中的微细胞在手术后发展出低活性分子表型.

Zhuoran Yin1,2, Anna K Leonard3, Carl M Porto3

  • 1Department of Neurology, Brigham and Women's Hospital, Boston, MA, USA.

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概括

手术暂时抑制老年小鼠的微质炎症反应,挑战了关于手术后脑部炎症的假设. 这项研究显示,在手术后,微质细胞,特别是老年动物的微质细胞,发生了恒常的转变.

关键词:
衰老的衰老 衰老的衰老微质细胞中的微质细胞神经炎症是一种神经炎症.外科手术 医疗外科手术

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科学领域:

  • 神经科学是一个神经科学.
  • 免疫学 免疫学 免疫学
  • 衰老研究研究 衰老研究

背景情况:

  • 微质细胞是关键的大脑免疫细胞,参与中枢神经系统平衡.
  • 失调的微质活动可能会导致神经炎症和神经退行.
  • 手术后的认知缺陷与炎症有关,微质因形态变化而受到影响.

研究的目的:

  • 为了研究手术对微质转录组的影响.
  • 测试外科手术诱导年龄依赖的亲炎性微质表型的假设.

主要方法:

  • 在年轻和老年小鼠的腹部腹腔切除术中,在手术后6小时和48小时进行牺牲.
  • 细胞因子分析 (ELISA),Iba1免疫组织化学和微质隔离通过FACS.
  • 使用qPCR和RNA测序进行转录组分析.

主要成果:

  • 手术增加了血细胞因子;CCL2在大脑中增加,在老年小鼠中更为如此.
  • 微细胞在手术后采用了恒温的分子现象型,特别是在老年小鼠中.
  • 手术上调的基因通常在神经退行性微质 (MGnD/DAM) 中下调,这种短暂的效果在48小时内消失.

结论:

  • 麻醉和手术会在外围引发暂时的炎症变化,但在中心引发一种恒常的微质表型.
  • 这种恒常的微质反应在老年小鼠中更为明显.
  • 这些发现挑战了外科手术始终在老年大脑中激活微质的观念.